| 柱前衍生—高效液相色谱法测定鱼粉中组胺的含量 |
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| 引用本文： | 万译文,;黄向荣,;黄华伟,;李小玲.柱前衍生—高效液相色谱法测定鱼粉中组胺的含量[J].湖南农业科学,2014(7):73-75. |
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| 作者姓名： | 万译文 ;黄向荣 ;黄华伟 ;李小玲 |
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| 作者单位： | [1]农业部渔业产品质量监督检验测试中心长沙,湖南长沙410153; [2]湖南省水产科学研究所,湖南长沙410153 |
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| 摘 要： | 为了建立鱼粉中组胺药物残留量测定的柱前衍生-高效液相色谱法,将样品匀浆后经高氯酸水溶液超声提取,提取液经丹酰氯衍生后,采用紫外检测器在254 nm处对组胺酮进行检测。结果表明,组胺分离效果好,检出限（LOD）为25.0 mg/kg,定量限（LOQ）为50.0μg/kg,相对标准偏差为2.11%~4.76%（n=6）,加标回收率达到79.5%~90.1%。该方法具有比较高的重现性和选择性,适用于鱼粉中组胺的残留测定。
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| 关 键 词： | 柱前衍生 高效液相色谱法 组胺 鱼粉 |
Determination of Histamine Residues in Fish Meal by High Performance Liquid Chromatography with Pre-Column Derivatization |
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| Institution: | WAN Yi-wen, HUANG Xiang-rong, HUANG Hua-wei, LI Xiao-ling(1. Fishery Products Quality Supervision and Testing Center, Ministry of Agriculture, Changsha 410153, PRC; 2. Hunan Institute of Fisheries Science, Changsha 410153, PRC) |
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| Abstract: | Established an analytical method for the determination of histamine in fish meal by high performance liquid chromatography with pre-column derivatization. The homogenated sample were ultrasonically extracted with perchloric acid aqueous solution, derivatized with dansylchloride,detected with a U V detectorat254 nmforhistamine.The resultshowed thatthe histamine can be separated efficiently.The detection limits of this method were 25.0μg/kg, and the limits of quantification were 50.0μg/kg, The relative standard deviations ranging from2.11% ~4.76% （n=6）and there covery rates were from 79.5% ~90.1% .The method proposed by this paper possesses higher prodicibility and selectivity,Itis promising in realapplications ofdetermining the histamine in fish meal. |
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| Keywords: | pre-column derivatization high performance liquid chromatography histamine fish meal |
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