| 11种杀虫剂对松墨天牛肌肉LIM蛋白基因表达的影响 |
| |
| 引用本文： | 罗淋淋,林同.11种杀虫剂对松墨天牛肌肉LIM蛋白基因表达的影响[J].湖南农业科学,2014(7):33-35. |
| |
| 作者姓名： | 罗淋淋 林同 |
| |
| 作者单位： | 华南农业大学林学院,广东广州510225 |
| |
| 基金项目： | 国家自然科学基金（31170612） |
| |
| 摘 要： | 松墨天牛（MonochamusalternatusHope）是松树的重要蛀干害虫,也是松材线虫（Bursaphelenchusxylophilus）的主要传播媒介。试验从松墨天牛文库中分离得到肌肉LIM蛋白基因的部分cDNA序列,长度为871 bp,命名为MaLIM（GenBank：KJ872589）;与赤拟谷盗（Tribolium castaneum）和家蚕（Bombyxmori）氨基酸相似性分别为77%和75%。用RT-qPCR测定了11种杀虫剂对MaLIM相对表达量的影响,结果表明,杀虫剂处理后MaLIM基因的表达量均有所下降,仅为对照的0.03~0.53。
|
| 关 键 词： | 松墨天牛 杀虫剂 肌肉蛋白基因 RT-qPCR |
Expression of Muscle LIM Protein Gene from Monochamus alternatus in Response to 11 Kinds of Pesticides |
| |
| LUO Lin-lin,LIN Tong.Expression of Muscle LIM Protein Gene from Monochamus alternatus in Response to 11 Kinds of Pesticides[J].Hunan Agricultural Sciences,2014(7):33-35. |
| |
| Authors: | LUO Lin-lin LIN Tong |
| |
| Institution: | (College of Forestry, South China Agricultural University, Guangzhou 510225, PRC) |
| |
| Abstract: | Monochamus alternatus H ope is one of the important pests of pine stemborers,and also is vector of pine wood nematode （Bursaphelenchusxylophilus）.Inthisstudy,apartialcDNA sequenceofmuscleLIMproteingene with870bpinlengthwasisolatedfromM.alternatuslibrary and named MaLIM（G enB ank：K J872589） thathas 77% and 75% amino acid similarity with the muscle L IMprotein of Triboliumcastaneumand Bombyx mori respectively. T he relative expression of MaLIMin response to 11 kinds of insecticides was determined by R T-qPC R ,the results showed thatthe MaLIMwas down regulated and the expression levelwas changed from0.03 times to 0.53 times of the control. |
| |
| Keywords: | Monochamus alternatus Hope pesticide muscle protein gene real time quantitative PCR |
| 本文献已被 维普 等数据库收录！ |
|
