| 重组多子小瓜虫抑动抗原ISCOMs制备 |
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| 引用本文： | 柯翎,陈如敬,刘晓东,杨金先,龚晖.重组多子小瓜虫抑动抗原ISCOMs制备[J].中国水产科学,2011,18(4):924-928. |
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| 作者姓名： | 柯翎 陈如敬 刘晓东 杨金先 龚晖 |
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| 作者单位： | 1. 福建省农业科学院生物技术研究所,福建福州,350003
2. 福建省农业科学院畜牧兽医研究所,福建福州,350013 |
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| 基金项目： | 福建省自然基金项目，福建省科技厅平台项目，福建省科技厅公益项目 |
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| 摘 要： | 提取表达于四膜虫(Tetrahymena thermophila)细胞膜表面的多子小瓜虫抑动抗原.通过Western Blotting证实重组多子小瓜虫抑动抗原约为35.7 kD,其免疫原性与多子小瓜虫抑动抗原相似.以Quil-A作为佐剂,采用离心、超声波和透析等方法制备小瓜虫抑动抗原免疫刺激复合物(Iag-ISCOMs).电镜观察可见Iag-ISCOMs经磷钨酸负染后呈直径30～40 nm的笼格状结构.免疫和攻毒试验证实Iag-ISCOMs对鳗鲡具有一定保护能力,攻毒后存活率达71.4％,对照组存活率为43.9％,结果表明,本实验成功制备了具有一定免疫原性的Iag-ISCOMs.
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| 关 键 词： | 小瓜虫 抑动抗原 免疫复合刺激物 |
| 修稿时间： | 2011/8/16 0:00:00 |
Preparation of recombinant immobilization antigen of Ichthyophthirius multifiliis |
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| KE Ling,CHEN Rujing,LIU Xiaodong,YANG Jinxian,GONG Hui.Preparation of recombinant immobilization antigen of Ichthyophthirius multifiliis [J].Journal of Fishery Sciences of China,2011,18(4):924-928. |
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| Authors: | KE Ling CHEN Rujing LIU Xiaodong YANG Jinxian GONG Hui |
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| Institution: | KE Ling1,CHEN Rujing2,LIU Xiaodong1,YANG Jinxian1,GONG Hui1 1.Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China,2.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fuzhou 350013 |
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| Abstract: | The recombinant immobilization antigen(Iag) of Ichthyophthirius multifiliis was extracted from mem-brane protein of Tetrahymena thermophila.The molecular weight of the recombinant immobilization antigen is 35.7 kD.In addition,western blotting corroborated that the recombinant Iag had similar immunogenic features to the Iag of I.multifiliis.Iag-ISCOMs were prepared by centrifugation,ultrasonic,dialysis and by using Quil-A as the adjuvant.Results from negative contrast electron microscopy observations indicat... |
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| Keywords: | Ichthyophthirius multifiliis immobilization antigen immunostimulating complex |
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