Quantitative changes in a serum protein,AS, during the life cycle of the lamprey,Petromyzon marinus L. |
| |
Authors: | Dr Michael F Filosa Misa A Ito John H Youson |
| |
Institution: | (1) Department of Zoology, University of Toronto, M1C 1A4 West Hill, Ontario, Canada;(2) Anatomy, Scarborough Campus, University of Toronto, M1C 1A4 West Hill, Ontario, Canada;(3) Division of Life Sciences, Scarborough Campus, University of Toronto, M1C 1A4 West Hill, Ontario, Canada |
| |
Abstract: | The electrophoretically fastest migrating protein in ammocoete serum, AS protein, was isolated and found to be an acidic glycoprotein with a molecular weight of 140,000 Daltons. Purified AS was injected into rabbits to produce a monospecific antiserum that was used in rocket immunoelectrophoresis to quantitate AS at different periods in the life cycle. AS was found to constitute approximately 70% of total serum protein in the ammocoete, remained at about the same value during transformation, and was reduced to 38% of total serum protein in the juvenile adult. In both male and female upstream migrant animals, AS was less than 0.5% of total serum protein. When juveniles were acclimated to seawater over a 10 week period, it was found that their AS level was reduced by 92% to 3.8% of total serum protein. These results are discussed in relation to changes in other serum proteins at various intervals of the lamprey life cycle. |
| |
Keywords: | lamprey AS serum protein protein purification monospecific antiserum |
本文献已被 SpringerLink 等数据库收录! |
|