A broad spectrum,one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2 |
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| Authors: | Shin-ichi Shimizu Takao Ito Takanori Miyoshi Yasunobu Tachibana Tsutae Ito |
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| Affiliation: | (1) Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan;(2) Present address: Ehime Prefectural Government, Matsuyama, Ehime, Japan;(3) Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), Akitsu Hiroshima, 739-2494, Japan;(4) Present address: Ehime Prefectural Toyo Regional Office Imabari Bureau, Imabari, Ehime, Japan;(5) Present address: Ehime Agricultural College, Matsuyama, Ehime, Japan;(6) Apple Research Station, NIFTS, Iwate, Japan; |
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| Abstract: | Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity. |
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