| 桑树毛状根生长动力学研究 |
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| 引用本文： | 杨金宏. 桑树毛状根生长动力学研究[J]. 中国农学通报, 2013, 29(1): 53-55. DOI: 10.11924/j.issn.1000-6850.2012-2844 |
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| 作者姓名： | 杨金宏 |
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| 作者单位： | 安康学院/陕西省蚕桑重点实验室,陕西安康,725000 |
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| 基金项目： | 陕西省教育厅科技计划项目“转家蚕attacin抗菌肽基因对桑树抗性的影响”(2010JK391);陕西省教育厅重点实验室项目“桑树毛状根的培养与综合利用”(2010JS011) |
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| 摘 要： | 为建立桑树毛状根的高效培养体系，采用液体摇瓶培养法，以培养30天的毛状根湿重为指标，对桑树毛状根的培养基及培养条件进行研究。结果表明，桑树毛状根的最佳培养基是pH 6.0的MS培养基，最优培养条件为26℃，130 r/min，暗培养。桑树毛状根的生长表现为“慢—快—慢”的趋势，呈“S”型曲线，生长21天左右进入稳定期。
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| 关 键 词： | 研究述评 研究述评 |
| 收稿时间： | 2012-08-16 |
| 修稿时间： | 2012-09-24 |
Study on the Growth Dynamics of Mulberry Hairy Roots |
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| Yang Jinhong. Study on the Growth Dynamics of Mulberry Hairy Roots[J]. Chinese Agricultural Science Bulletin, 2013, 29(1): 53-55. DOI: 10.11924/j.issn.1000-6850.2012-2844 |
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| Authors: | Yang Jinhong |
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| Affiliation: | Yang Jinhong(Ankang University /Key Sericultural Laboratory of Shaanxi,Ankang Shaanxi 725000) |
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| Abstract: | In order to establish the high efficient culture system of mulberry hairy root, the medium formula and culture conditions of mulberry hairy roots in submerged culture with shake flask were studied according to the index of the wet weight cultured 30 d. The content of total flavonoids in mulberry hairy roots was determined by spectrophotometry using rutin as standard substance. The results showed that the optimum medium was pH 6.0 MS culture medium, and the optimum culture condition was 26℃, 130 r/min, dark culture. The growth trends of mulberry hairy roots was“slow-fast-slow”, as“S”type curve, and the stable period was after cultured 21 days. |
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| Keywords: | growth dynamics |
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