橡胶树白粉病菌分生孢子在不同介质的萌发过程及其内容物的变化

 本研究采用不同的染色方法和显微技术观察橡胶树白粉病菌(Oidium heveae)分生孢子的超微结构，分生孢子在不同介质表面及不同介质溶液中的萌发情况，以及孢子萌发过程中孢内主要物质的变化，以明确O. heveae分生孢子在不同介质萌发的形态变化及萌发所需能量来源。结果表明：O. heveae 分生孢子表面具有较浅的花纹结构，呈椭圆形或者卵圆形，大小为26.1~45.1μm×13.5~21.9μm。O. heveae 分生孢子在亲水和疏水介质上均能萌发产生芽管和附着胞，在葡萄糖水中的萌发率略高于清水中，但萌发率无显著性差异 (P>0.05)，芽管和附着胞形成与形态无明显差别。分生孢子萌发过程中，几个液泡会融合形成一个，最终消散而呈不明显可见的泡囊结构。孢子内的糖原、脂质及分裂后的核仁等可通过芽管向附着胞输送，表明O. heveae 分生孢子萌发所需能量物质主要来源于自身能量贮备。

Germination morphology of conidia of Oidium heveae in different media and its content changes

By using various staining methods, the germination process and phenotype changes of conidia of rubber tree powdery mildew (Oidium heveae) on different substrates were observed. The aim is to investigate the conidia phenotype changes on the surface of different media and the energy source needed for spore germination and apressorium formation. Conidia of O. heveae are oval or nearly spherical shape, 26.1~45.1 μm×13.5~21.9μm in size. Spore germination and appressorium formation were not influenced by the contact surfaces, or by hydrophilic and hydrophobic media. The shape of germ tube and appressorium was not significantly different in different media (P>0.05). During conidium germination, several vacuoles fused into one and gradually dissipated so that no obvious vacuole structure could be observed. The conidia of O. heveae could germinate in water and glucose solution. Glycogen and lipids stored in conidia could be transferred to appressorium via germ tube during conidia germination, and nucleolus could also be transferred to appressorium. The results demonstrated that energy for germination came from conidia’s own glycogen and lipid reserves.