| 2苹果品种叶片胚状体再生体系的建立 |
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| 引用本文： | 魏海霞1,刘德玺1,王 霞1,周 健1,王莉莉1,刘 丹2,祝 军3. 2苹果品种叶片胚状体再生体系的建立[J]. 西北林学院学报, 2014, 29(1): 84-88. DOI: 10.3969/j.issn.1001-7461.2014.01.17 |
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| 作者姓名： | 魏海霞1 刘德玺1 王 霞1 周 健1 王莉莉1 刘 丹2 祝 军3 |
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| 作者单位： | 1.山东省林业科学研究院,山东 济南 250014; 2.山东省林木种质资源中心,山东 济南 250014; 3.青岛农业大学,山东 青岛 266109 |
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| 摘 要： | 以苹果“富士”和“鲁加6号”为材料,建立叶片胚状体再生体系。结果表明:先经胚性细胞诱导培养基MS+BA1.0 mg·L-1+NAA5.0 mg·L-1+TDZ0.5 mg·L-1诱导胚性细胞,再置于胚胎发育培养基MS+BA1.0 mg·L-1+NAA0.1 mg·L-1上进行暗培养,最佳时间为10 d,2品种叶片再生率分别为96.7%和100%。经胚状体不同时期形态学组织学观察,显示胚状体发育时期经历原胚、球形胚、心形胚、子叶形胚、鱼雷形胚、成熟胚等阶段。“鲁加六号”苹果最佳生根培养基为1/2MS+IAA0.1 mg·L-1+IBA0.1 mg·L-1,生根率达86.7%。
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| 关 键 词： | 胚状体 再生 形态学 组织学 苹果 |
Establishment of Regeneration System of Embryoid from the Leaves of Two Apple Varieties |
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| WEI Hai-xia1,LIU De-xi1,WANG Xia1,ZHOU Jian1,WAND Li-li1,LIU Dan2,ZHU Jun3. Establishment of Regeneration System of Embryoid from the Leaves of Two Apple Varieties[J]. Journal of Northwest Forestry University, 2014, 29(1): 84-88. DOI: 10.3969/j.issn.1001-7461.2014.01.17 |
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| Authors: | WEI Hai-xia1 LIU De-xi1 WANG Xia1 ZHOU Jian1 WAND Li-li1 LIU Dan2 ZHU Jun3 |
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| Affiliation: | 1. Shandong Academy of Forestry, Ji’nan, Shandong 250014, China; 2. Shandong Forest Tree Germplasm Resources Center,Ji’nan, Shandong 250014, China; 3. Qingdao Agricultural University, Qingdao, Shandong 266109, China |
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| Abstract: | Regeneration systems of 2 apple cultivars(Fuji and Lujia 6)were established by using leaf embryoids. First, embryonic cells were induced in the medium of MS+BA1.0 mg·L-1+NAA5.0 mg·L-1+TDZ0.5 mg·L-1,and then the resultant cells were cultured in the dark in MS+BA1.0 mg·L-1+NAA0.1 mg·L-1 with an optimal culture time of 10 d.The rates of leaf regeneration of 2 cultivars were 96.7% and 100%, respectively. Morphological and histological observations demonstrated that embryoids underwent different developmental periods: from original embryo to globular,cordiform,cotyledonary, fish torpedo-shaped, and mature embryos. The optimal rooting medium for ‘Lujia-6’ was obtained as 1/2MS+IAA0.1 mg·L-1+IBA0.1 mg·L-1,by which the rooting rate was 86.7%. |
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| Keywords: | embryoid regeneration morphology histology apple |
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