2苹果品种叶片胚状体再生体系的建立

以苹果“富士”和“鲁加6号”为材料,建立叶片胚状体再生体系。结果表明:先经胚性细胞诱导培养基MS+BA1.0 mg·L^-1+NAA5.0 mg·L^-1+TDZ0.5 mg·L^-1诱导胚性细胞,再置于胚胎发育培养基MS+BA1.0 mg·L^-1+NAA0.1 mg·L^-1上进行暗培养,最佳时间为10 d,2品种叶片再生率分别为96.7%和100%。经胚状体不同时期形态学组织学观察,显示胚状体发育时期经历原胚、球形胚、心形胚、子叶形胚、鱼雷形胚、成熟胚等阶段。“鲁加六号”苹果最佳生根培养基为1/2MS+IAA0.1 mg·L^-1+IBA0.1 mg·L^-1,生根率达86.7%。

Establishment of Regeneration System of Embryoid from the Leaves of Two Apple Varieties

Regeneration systems of 2 apple cultivars(Fuji and Lujia 6)were established by using leaf embryoids. First, embryonic cells were induced in the medium of MS+BA1.0 mg·L^-1+NAA5.0 mg·L^-1+TDZ0.5 mg·L^-1,and then the resultant cells were cultured in the dark in MS+BA1.0 mg·L^-1+NAA0.1 mg·L^-1 with an optimal culture time of 10 d.The rates of leaf regeneration of 2 cultivars were 96.7% and 100%, respectively. Morphological and histological observations demonstrated that embryoids underwent different developmental periods: from original embryo to globular,cordiform,cotyledonary, fish torpedo-shaped, and mature embryos. The optimal rooting medium for ‘Lujia-6’ was obtained as 1/2MS+IAA0.1 mg·L^-1+IBA0.1 mg·L^-1,by which the rooting rate was 86.7%.