2010—2013年浙江省猪流行性腹泻病毒临床检测及PEDV S基因型分析

2010年以来，全国大部分地区暴发新生仔猪严重流行性腹泻，给养猪业造成巨大经济损失。为分析猪流行性腹泻病毒(PEDV)在浙江省的流行和遗传变异情况，对2010—2013年收集的临床腹泻样品，以针对PEDV S基因N端序列的RT PCR检测病原，并测序其中的41份样品的扩增序列，分析PEDV S1基因型变异情况。临床检测结果表明：　PEDV临床检出率由2010年的4286%提高到2013年的70%以上，并且浙江省各地的检出率都在50%以上；一年中7至8月份检出率明显降低。 PEDV S1基因N端序列分析结果表明，临床检测株的S1基因与传统毒株PEDV CV777的S基因序列及中国最早检测到的基因序列亲缘关系较远，可能浙江省PEDV的流行株可能属于一个新的基因型。同时研究结果提示PEDV S基因的变异可能是现有CV777毒株疫苗免疫保护力不佳的原因。

Genotyping and clinical detection of porcine epidemic diarrhea virus during 2010-2013 in Zhejiang Province

Since 2010， porcine epidemic diarrhea in newborn piglets seriously outbroke in most parts of China and caused huge economic losses to the pig industry. For the analysis of epidemic and genetic variation of porcine epidemic diarrhea virus (PEDV) in Zhejiang Province， PEDV in clinical samples collected during 2010-2013 was detected by RT PCR based on N terminal of S gene， and fragments of 41 sequences were analyzed for variation of S1 gene. The results showed that the PEDV clinical detection rate increased from 4286% in 2010 to over 70% in 2013. The detection rate was significantly lower in July and August and was more than 50% across Zhejiang province. Sequencing analysis showed that the S gene of clinical detected strains were heterogenous with that of PEDV CV777， indicating that the current epidemic strain probably makes a new genotype and the mutation of PEDV S1 gene may be the reason for low immune protection of the existing CV777 strain vaccine．