| Folin-Ciocalteu法测定大熊猫主食竹中多酚含量 |
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| 引用本文： | 赵莘,段丽娟,高健,郑丽丽,李俊清. Folin-Ciocalteu法测定大熊猫主食竹中多酚含量[J]. 西北林学院学报, 2014, 29(6): 212-216. DOI: doi:10.3969/j.issn.1001-7461.2014.06.40 |
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| 作者姓名： | 赵莘 段丽娟 高健 郑丽丽 李俊清 |
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| 作者单位： | （北京林业大学 省部共建森林培育与保护教育部重点实验室，北京 100083） |
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| 摘 要： | 为建立 Folin-Ciocalteu法测定大熊猫主食竹缺苞箭竹(Fargesia denudata)总酚酸含量的方法。以没食子酸为对照品，研究了Folin-Ciocalteu法测定缺苞箭竹中酚酸类物质含量的适宜条件。结果表明，适宜的比色条件为Folin-Ciocalteu试剂与 10% Na2CO3体积比为1∶6，Folin-Ciocalteu试剂3 mL，显色温度25℃，反应时间2 h，检测波长780 nm。浓度为20～100 μg·mL-1时与其吸光值呈良好的线性关系，测定方法的平均回收率为99.4%。可见该法有较好的准确度和重现性。
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| 关 键 词： | 缺苞箭竹 Folin-Ciocalteu法 总酚 |
Determination of the Contents of Polyphenols in Fargesia denudata, the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method |
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| ZHAO Shen,DUAN Li-juan,Gao Jian,ZHENG Li-li,LI Jun-qing. Determination of the Contents of Polyphenols in Fargesia denudata, the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method[J]. Journal of Northwest Forestry University, 2014, 29(6): 212-216. DOI: doi:10.3969/j.issn.1001-7461.2014.06.40 |
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| Authors: | ZHAO Shen DUAN Li-juan Gao Jian ZHENG Li-li LI Jun-qing |
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| Affiliation: | (The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China) |
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| Abstract: | Aims: to establish a quantitative method of measuring total phenols in Fargesia denudata, the main food of giant panda. Methods: with gallic acid as reference substance to study the suitable conditions of the measurement by Folin-Ciocalteu method. Results: the optimum conditions for determining total polyphenols were as follows: the volume ratio of Folin-Ciocalteu reagent to 10% Na2CO3: 1∶6, 3 mL of Folin -Ciocalteu reagent incubated at 25℃for 2 h, followed by detection at 780 nm. Good linear relationship was found in the concentration of 20-100 μg·mL-1, the average recovery was 99.4%. |
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| Keywords: | Fargesia denudataFargesia denudata Folin-Ciocalteu ployphenol |
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