| 超声辅助提取法对黄芩总黄酮含量的影响 |
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| 引用本文： | 阿木古楞,裴乐,李锋,耿万恒,侯勇跃,卢岩,李子健,刘秀丽.超声辅助提取法对黄芩总黄酮含量的影响[J].畜牧与饲料科学,2020,41(1):73-76. |
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| 作者姓名： | 阿木古楞 裴乐 李锋 耿万恒 侯勇跃 卢岩 李子健 刘秀丽 |
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| 作者单位： | 内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031 |
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| 基金项目： | 2018年内蒙古科技厅创新引导项目（KCBJ2018062）;国家重点研发计划项目—政府间国际科技创新合作重点专项（2017YFE0108400）;内蒙古自治区科技计划重大专项项目 |
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| 摘 要： | 为从不同超声条件中,筛选出黄芩总黄酮的最佳提取工艺。用超声波辅助技术提取黄芩总黄酮,用亚硝酸钠—硝酸铝比色法测定总黄酮含量。通过单因素和正交试验,确定了黄芩总黄酮的最佳提取工艺为乙醇浓度为50%,超声处理时间为25 min,料液比1∶25,超声处理温度为70 ℃。在该条件下,总黄酮提取量为15.15 mg/g。
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| 关 键 词： | 黄芩 总黄酮 超声辅助 正交试验 |
| 收稿时间： | 2019-12-05 |
Effect of Ultrasound-assisted Extraction on Total Flavonoids Content in Radix scutellariae |
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| Amuguleng,PEI Le,LI Feng,GENG Wan-heng,HOU Yong-yue,LU Yan,LI Zi-jian,LIU Xiu-li.Effect of Ultrasound-assisted Extraction on Total Flavonoids Content in Radix scutellariae[J].Animal Husbandry and Feed Science,2020,41(1):73-76. |
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| Authors: | Amuguleng PEI Le LI Feng GENG Wan-heng HOU Yong-yue LU Yan LI Zi-jian LIU Xiu-li |
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| Institution: | Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China |
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| Abstract: | The objective of this study was to determine the optimum ultrasound-assisted extraction condition for total flavonoids from Radix scutellariae.Extraction of total flavonoids from Radix scutellariae were carried out with ultrasonic-assisted technology,and the total flavonoids content was determined using sodium nitrite-aluminum nitrate colorimetric method.Via single factor and orthogonal experiments,the optimum extraction process of total flavonoids from Radix scutellariae was determined as followed:ethanol concentration was 50%,sonication time was 25 min,material-liquid ratio was 1∶25,and sonication temperature was 70℃.Under these conditions,the obtained total flavonoid extraction quantity was 15.15 mg/g. |
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| Keywords: | Radix scutellariae total flavonoids ultrasound assistance orthogonal test |
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