Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand |
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| Authors: | Kiatipattanasakul-Banlunara Wijit Tantilertcharoen Rachod Suzuki Kazuhiko Albarenque Stella Maris Thanawongnuwech Roongroje Nakayama Hiroyuki Doi Kunio |
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| Institution: | Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. |
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| Abstract: | A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III. |
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