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Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand
Authors:Kiatipattanasakul-Banlunara Wijit  Tantilertcharoen Rachod  Suzuki Kazuhiko  Albarenque Stella Maris  Thanawongnuwech Roongroje  Nakayama Hiroyuki  Doi Kunio
Institution:Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Abstract:A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.
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