| 利用ISSR标记对12种五针松亲缘关系的研究 |
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| 引用本文： | 刘桂丰,董京祥,姜莹,吕艳芳,姜静,赵光仪. 利用ISSR标记对12种五针松亲缘关系的研究[J]. 林业研究, 2005, 16(3) |
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| 作者姓名： | 刘桂丰 董京祥 姜莹 吕艳芳 姜静 赵光仪 |
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| 作者单位： | 东北林业大学林学院，东北林业大学林学院，东北林业大学林学院，东北林业大学林学院，东北林业大学林学院，东北林业大学林学院 哈尔滨 150040，哈尔滨 150040，哈尔滨 150040，哈尔滨 150040，哈尔滨 150040，哈尔滨 150040 |
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| 基金项目： | The study was supported by Introducing Overseas Ad-vanced Agriculture Science and Technology Program of China (948 Program) (2001-31) |
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| 摘 要： | 采用ISSR 标记技术,对12 种五针松的亲缘关系进行分析。利用筛选出的12 个ISSR 引物共检测到117 个位点,多态条带比率(PPB)在9.40%～33.33 %之间,遗传分化最高的是偃松,最低的是柔枝松。12 种五针松的基因多样性(Ht)为26.21%,其中种内基因多样性(Hs)为7.66%,种间基因多样性(Dst)为18.55%,五针松种间变异占总变异的70.78%。遗传距离聚类,将12 种五针松分为2 个类群,第一类群包括乔松、华山松、海南五针松、华南五针松、北美乔松、山白松和云南五针松;第二类群包括美国白皮松、偃松、、柔枝松、西伯利亚红松和红松。图1 表3 参6。
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| 关 键 词： | 五针松 ISSR-PCR 亲缘关系 |
Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers |
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| LIU Gui-feng,DONG Jing-xiang,JIANG Ying,LU Yan-fang,JIANG Jing,ZHAO Guang-yi Forestry College,Northeast Forestry University,Harbin ,P. R. China. Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers[J]. Journal of Forestry Research, 2005, 16(3) |
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| Authors: | LIU Gui-feng DONG Jing-xiang JIANG Ying LU Yan-fang JIANG Jing ZHAO Guang-yi Forestry College Northeast Forestry University Harbin P. R. China |
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| Affiliation: | LIU Gui-feng,DONG Jing-xiang,JIANG Ying,LU Yan-fang,JIANG Jing,ZHAO Guang-yi Forestry College,Northeast Forestry University,Harbin 150040,P. R. China |
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| Abstract: | Genetic relationship of 12 species of Section Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%. P. pumila had the highest levels of genetic differentiation and P. flexilis had lowest. Total genetic diversity (HT) of 12 species in Section Strobus was 26.21%, of which intraspecific genetic diversity (HS) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group in- cluded P. griffithii, P. armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola and P. wangii. The second group included P. albi- caulis, P. pumila, P. flexilis, P. sibirica and P. koraiensis. |
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| Keywords: | Pinus ISSR-PCR Genetic relationship |
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