高效液相色谱法测定芩黄颗粒中甘草酸的含量

为了建立芩黄颗粒中甘草酸的含量测定方法并测定其含量，用高效液相色谱法（HPLC）测定芩黄颗粒中甘草酸的含量。采用C18色谱柱，以甲醇-0．2mol／L醋酸铵（65：34，醋酸调节pH值至4．5）为流动相，流速为1．0mL／min，检测波长为250nm。结果显示甘草酸在0．499～7．976μg之间呈现良好的线性关系，r=0．9999，平均加样回收率为99．3％，RSD=0．59％（n=6）。本方法重复性好，能准确测定芩黄颗粒中甘草酸的含量。

Determination of Glycyrrhizic acid in Qinhuang Granula by HPLC

To establish the method for determining of glycyrrhizic acid in Qinhuang Granula by HPLC, C18 column was used with the mobile phase consisted of methanol-0.2mol/L ammonium acetate （65 ： 34, adjusted pH to 4.5 with CH3COOH）, the flow rate was 1.0 mL/min, the detection wavelength was 250 nm. The calibration curve was linear at a range of 0.499 - 7. 977 μg for glycyrrhizic acid （ r = 0.9999 ） , the average recovery was 99.3%and RSD was 0.59% （n= 6）. The method was accurate with a good reproducibility and can be used as a quantitative analysis of glycyrrhizic acid in Qinhuang Granula.