Identification of SSR loci in Betula luminifera using birch EST data |
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| Authors: | Yong-quan Lu Hai-ying Li Qing Jia Hua-hong Huang Zai-kang Tong |
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| Affiliation: | LU Yong-quan LI Hai-ying JIA Qing HUANG Hua-hong TONG Zai-kangThe Nurturing Station for the State Key Laboratory of Subtropitical Silviculture Zhejiang Agriculture and Forestry University Lin'an 311300,China. |
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| Abstract: | Expressed sequence tags(ESTs) are generated from single-pass sequencing of randomly picked cDNA clones and can be used for development of simple sequence repeat(SSR) markers or microsatellites.However,EST databases have been developed for only a small number of species.This paper provides a case study of the utility of freely available birch EST resources for the development of markers necessary for the genetic analysis of Betula luminifera.Based on birch EST data,primers for 80 EST-SSR candidate loci were ... |
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| Keywords: | exploit Betula luminifera birch EST database EST-SSR |
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