秦艽组织培养研究

目的]为解决中药秦艽的种源问题。方法]以秦艽的幼嫩叶片为外植体,在不同培养基上进行愈伤组织的诱导和分化以及根的诱导,寻找秦艽愈伤组织培养和快速繁殖的最佳途径。结果]秦艽愈伤组织培养和快速繁殖的最佳途径为:以秦艽幼嫩叶片为外植体,以MS+1.0 mg/L NAA+1.0 mg/L 6-BA为愈伤组织的诱导培养基,以MS+0.3 mg/L NAA+1.0 mg/L 6-BA为愈伤组织的分化培养基,以1/2 MS为生根培养基。通过该途径,可在较短周期内得到再生的秦艽植株。结论]该研究结果可用于秦艽的生物技术育种,并能推动秦艽的人工栽培。

Prelinfinary Study on Tissue Culture of Gentiana macrophylla Pall.

Objective] The research aimed to solve the seed source problem of traditional Chinese medicine Gentiana macrophylla Pall.Method] With the tender leaves of G.macrophylla as explants,the callus induction and differentiation,and the root induction were conducted on different medium to seek the optimum approach for the tissue culture and rapid propagation of G.macrophylla.Result] The optimum approaches for the tissue culture and rapid propagation of G.macrophylla were as follows: taking the tender leaves of G.macrophylla as explants,taking MS 1.0 mg/L NAA 1.0 mg/L 6-BA as the induction medium of callus,taking MS 0.3 mg/L NAA 1.0 mg/L 6-BA as the differentiation medium of callus and taking 1/2 MS as the rooting medium.Through this approach,the regenerated plants could be gained in a shorter period.Conclusion] The research results could be applied in the biotechnology breeding of G.macrophylla and promote the artificial cultivation of G.macrophylla.