Establishment of bipotent progenitor cell clone from rat skeletal muscle |
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| Authors: | Yousuke MURAKAMI Erica YADA Shin‐ichi NAKANO Yuko MIYAGOE‐SUZUKI Tohru HOSOYAMA Takashi MATSUWAKI Keitaro YAMANOUCHI Masugi NISHIHARA |
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| Institution: | 1. Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo;2. Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Japan |
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| Abstract: | The present study describes the isolation, cloning and characterization of adipogenic progenitor cells from rat skeletal muscle. Among the obtained 10 clones, the most highly adipogenic progenitor, 2G11 cells, were further characterized. In addition to their adipogenicity, 2G11 cells retain myogenic potential as revealed by formation of multinucleated myotubes when co‐cultured with myoblasts. 2G11 cells were resistant to an inhibitory effect of basic fibroblast growth factor on adipogenesis, while adipogenesis of widely used preadipogenic cell line, 3T3‐L1 cells, was suppressed almost completely by the same treatment. In vivo transplantation experiments revealed that 2G11 cells are able to possess both adipogenicity and myogenicity in vivo. These results indicate the presence of bipotent progenitor cells in rat skeletal muscle, and suggest that such cells may contribute to ectopic fat formation in skeletal muscle. |
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| Keywords: | adipogenesis differentiation fibroblast growth factor myogenesis skeletal muscle |
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