猪繁殖与呼吸综合征病毒感染抑制猪瘟疫苗的免疫应答 |
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引用本文: | 李华,杨汉春,黄芳芳,刘平黄,高云,陈艳红. 猪繁殖与呼吸综合征病毒感染抑制猪瘟疫苗的免疫应答[J]. 中国兽医学报, 2001, 21(3): 219-222 |
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作者姓名: | 李华 杨汉春 黄芳芳 刘平黄 高云 陈艳红 |
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作者单位: | 中国农业大学动物医学院, |
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基金项目: | 国家自然科学基金资助项目(39870586) |
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摘 要: | 对20日龄SPF猪和20日龄猪繁殖与呼吸综合征(PRRS)血清阳性猪,人工感染猪繁殖与呼吸综合征病毒(PRRSV)北京分离株(BJ-4)后48h接种猪瘟疫苗,利用ELISA方法检测仔猪针对PRRSV和猪瘟疫苗的体液免疫,利用MTS法检测仔猪外周血单核细胞对有丝分裂原ConA的刺激反应。结果表明,不论是SPF仔猪还是带有PRRSV抗体的仔猪,在鼻内接种PRRSV后48h接种猪瘟疫苗,其对猪瘟疫苗的抗体反应显著低于对照组,对有丝分裂原ConA的刺激反应也下降。由此说明,PRRSV感染使仔猪对猪瘟疫苗的免疫应答受到抑制。
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关 键 词: | 猪 繁殖呼吸综合征病毒 猪瘟疫苗 免疫抑制 人工感染 |
文章编号: | 1005-4545(2001)03-0219-04 |
修稿时间: | 2001-02-14 |
Infection of Porcine Reproductive and Respiratory Syndrome Virus Inhibits Immune Response Against Hog Cholera Virus Vaccine |
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Abstract: | To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.Results of serum antibodies detected by ELISA indicated that the infected piglets had specific antibody against PRRSV BJ 4 strain in two weeks,and had significantly lower levels of the antibody against hog cholera vaccine than those of controls.Peripheral blood mononuclear cell(PBMC) from piglets infected with BJ 4 strain showed decreased proliferation responses to mitogen ConA.All results above indicated PRRS virus can suppress both the humoral and cellular immune responses of infected piglets. |
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Keywords: | PRRSV hog cholera vaccine immunosuppression |
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