害虫对Bt杀虫蛋白抗性相关受体蛋白互作网络分析

为对Bt杀虫蛋白与化学杀虫剂的协同使用提供理论指导，通过蛋白互作分析方法，以黑腹果蝇Drosophila melanogaster为模型，对化学杀虫剂与Bt杀虫剂作用受体以及抗性相关蛋白的互作网络进行研究。结果表明，Bt杀虫蛋白的27个受体在黑腹果蝇中共存在50个互作蛋白，其中有39个蛋白只与Bt杀虫蛋白抗性相关，11个蛋白还与化学杀虫剂抗性相关，这2类杀虫剂抗性互作网络交集较小，并且重叠蛋白的变异引起交互抗性的概率不大。表明Bt杀虫蛋白与化学杀虫剂产生交互抗性的概率较小，可以将Bt杀虫剂与化学杀虫剂协同使用，提高害虫防治效果，并有效克服或延缓害虫抗性产生。

Network analysis of proteins interacting with receptors related to resistance to Bacillus thuringiensis pesticidal proteins

In order to understand the mechanisms in resistance to Bacillus thuringiensis (Bt) pesticidal proteins and chemical insecticides, this study investigated the protein-interaction network among chemical insecticide receptors and Bt bioinsecticide receptors by using protein interaction analysis method with Drosophila melanogaster as a model. A total of 50 proteins interacting with 27 Bt pesticidal protein receptors were identified in D. melanogaster, of which 39 proteins were only related to Bt protein resistance, 11 proteins were also related to the chemical insecticide resistance. The intersection of these two networks was small, and the variation of overlapping proteins had little chance of causing cross-resistance. This study indicated that Bt pesticidal proteins and chemical insecticides had little chance to produce cross-resistance, and Bt bioinsecticide could be used synergistically with chemical insecticides to improve pest control effect and effectively overcome or delay the development of insecticide resistance.