| 地涌金莲的离体培养与快速繁殖技术研究 |
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| 引用本文： | 刘秀贤,潘庆杰,李正红,马宏,罗栋,万友名.地涌金莲的离体培养与快速繁殖技术研究[J].安徽农业科学,2010,38(9):4442-4444. |
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| 作者姓名： | 刘秀贤 潘庆杰 李正红 马宏 罗栋 万友名 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,云南昆明,650224;山东省胶州市少海发展管理处,山东胶州,266300 |
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| 基金项目： | 国家林业局林业公益性行业科研专项(200904053);;云南省应用基础专项(2009ZC188M) |
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| 摘 要： | 目的]研究地涌金莲（Musella lasiocarpa）的离体培养与快速繁殖技术。方法]以地涌金莲吸芽为外植体,诱导不定芽分化、增殖和生根。结果]适宜不定芽增殖的培养基为改良MS＋6-BA3.0mg/L＋NAA0.3mg/L＋AC2.0g/L＋Vc0.1g/L＋蔗糖30.0g/L＋琼脂8.0g/L,增殖系数为4.89;适宜生根的培养基为1/4改良MS＋IBA0.5mg/L＋蔗糖20.0g/L＋琼脂8.0g/L,生根率达100.00%,平均生根数4.60条,平均根长3.40cm;幼苗移栽成活率达91%。结论]为大面积推广应用地涌金莲奠定了基础。
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| 关 键 词： | 地涌金莲 离体培养 快速繁殖 |
Study on in vitro Culture and Rapid Propagation Technology of Musella lasiocarpa |
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| LIU Xiu-xian et al.Study on in vitro Culture and Rapid Propagation Technology of Musella lasiocarpa[J].Journal of Anhui Agricultural Sciences,2010,38(9):4442-4444. |
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| Authors: | LIU Xiu-xian |
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| Institution: | Research Institute of Resource Insect;CAF;Kunming;Yunnan 650224 |
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| Abstract: | Objective] The research aimed to study the in vitro culture and rapid propagation technology of Musella lasiocarpa. Method] The surculus sucker from Musella lasiocarpa were used as explants to induce buds and rooting in different culture media. Result] For its buds propagation,the optimum culture medium was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+AC 2.0 g/L+Vc 0.1 g/L+ sugar 30 g/L+ agar 8 g/L,with reproduction coefficient 4.89. The optimum culture medium for rooting was 1/4 MS+IBA 0.5 mg/L + sugar 20 g/L+ agar 8 ... |
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| Keywords: | Musella lasiocarpa in vitro culture Rapid propagation |
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