| HPLC-ESI-MS/MS检测蜂蜜中林可霉素残留 |
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| 引用本文： | 阮祥春,曾明华,赵明. HPLC-ESI-MS/MS检测蜂蜜中林可霉素残留[J]. 安徽农业科学, 2009, 37(5) |
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| 作者姓名： | 阮祥春 曾明华 赵明 |
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| 作者单位： | 安徽农业大学动物科技学院,安徽合肥,230036;安徽省兽药饲料监察所,安徽合肥,230022 |
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| 摘 要： | [目的]建立蜂蜜中林可霉素残留的高效液相色谱-电喷雾串联质谱(HPLC-ESI-MS/MS)检测方法。[方法]样品经固相萃取净化、液相色谱分离后进行质谱分析,在多反应监测模式(MRM)下进行特征母离子、子离子对信号采集。根据保留时间、母离子和两个特征子离子进行定性分析,以离子峰度最强的基峰离子m/z126进行定量。[结果]在1.0~20.0 ng/ml线性范围内,峰强度与添加浓度的线性关系良好(r2>0.9996)。在2.5、5.01、0.0 ng/g 3个添加水平,林可霉素的平均回收率范围为(95.1±3.3)%,日内测定结果的相对标准偏差小于5.3%,日间测定结果的相对标准偏差小于8.5%,最低检测限0.1 ng/g。[结论]HPLC-ESI-MS/MS用作检测蜂蜜中林可霉素残留的方法,灵敏度高、精密度好,达到了林可霉素残留分析的要求。
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| 关 键 词： | 蜂蜜 林可霉索 高效液相色谱-电喷雾串联质谱 残留 |
Detection on the Residues of Lincomycin in Honey by LC-ESI-MS/MS |
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| RUAN Xiang-chun et al. Detection on the Residues of Lincomycin in Honey by LC-ESI-MS/MS[J]. Journal of Anhui Agricultural Sciences, 2009, 37(5) |
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| Authors: | RUAN Xiang-chun et al |
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| Affiliation: | RUAN Xiang-chun et al (College of Animal Sciences,Anhui Agricultural University,Hefei,Anhui 230036) |
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| Abstract: | [Objective] The purpose was to set up the method of detecting the residues of lincomycin in honey by HPLC-ESI-MS/MS.[Method] The samples purified by solid-phase extraction and isolated by liquid-phase extraction were analyzed by MS.The signal collection of characteristic maternal ions and sub-ion pairs was conducted in MRM.The qualitative analysis was conducted on the basis of retaining time,maternal ions and the two characteristic sub-ions.The quantification was conducted on base-peak ion m/z126,whose ion ... |
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| Keywords: | Honey Lincomycin HPLC-ESI-MS/MS Residues |
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