《新编拉汉英植物名称》王宗训主编

The cardiac protection of non-mitogenic human fibroblast growth factor on cardiomyocytes treated with hydrogen peroxide in vitro

AIM: To study the protective effects of non-mitogenic human fibroblast growth factor (nm-haFGF) on cardiomyocytes injured by reactive oxygen free radicals. METHODS: The cardiomyocytes were isolated from neonatal SD mouse by trypsin digestion. The cardiomyocytes injury model was established by exposing the cells to hydrogen peroxide (H2O2), and the injury status in cardiomyocytes were evaluated by examining the cellular viability, measuring cell apoptosis and observing the change of cellular morphology. nm-haFGF was added to the culture medium, and the changes of cellular viability, superoxide dismutase (SOD), malondialdehyde (MDA) and celluar apoptosis were observed. RESULTS: A dose-dependence relation between the concentration of H2O2 and the cardiomyocytes injury was observed. 10-80 μg/L nm-haFGF dose-dependently increased cardiomyocyte viability and the general SOD activity, as well as decreased the content of MDA and the quantity of cardiomyocyte apoptosis. CONCLUSION: The higher the concentration of H2O2, the more serious the cardiomyocyte injury. nm-haFGF may have a good protective effects on cardiomyocytes treated with H2O2.