16个水稻品种稻米贮藏蛋白组分比较研究

选取16个水稻品种,其中包括籼稻品种4个(9311、明恢63、轮回422、kasalath)、粳型三系恢复系水稻品种4个(湘晴、C2、C9、C15)以及8个粳型常规稻、三系保持系或两系恢复系水稻(日本晴、越光、武香075、上师4号、寒丰、秀水09、嘉花1号、优丰),采用不连续的SDS-PAGE电泳分析16种水稻糙米和精米种子贮藏蛋白组分。各种水稻能够显示清晰的蛋白组分条带主要有:谷蛋白37~39 kD酸性亚基、22~23 kD碱性亚基、谷蛋白57 kD前体多肽和13 kD醇溶蛋白多肽。通过对不同水稻品种蛋白组分的分析,筛选到谷蛋白含量相对较高的籼稻和粳型材料。开展本试验可为今后利用杂交方法培育高谷蛋白常规水稻以及高谷蛋白杂交稻组合研究中的亲本选择提供参考。

Study on Comparison of Sixteen Varieties of Rice's Storage Protein

In this study we chose sixteen varieties of rice including four kinds of Indica rice(9311,Minghui63,Lunhui422,Kasalath)and four restorer lines for the three-line hybrid japonica rice(Xiangqing,C2,C9,C15),and eight Japonica conventional rice,or maintainer lines for three-line hybrid rice or male parents for two-line hybrid rice(Nipponbare,Koshihikari,Wuxiang075,Shangshi No.4,Hanfeng,Xiushui09,Jiahua No.1,Youfeng).Using discontinuous SDS-PAGE,storage protein comparisons in the brown rice and the polished rice of the sixteen varieties of rice were analysed.The 57kD precursor,37-39kD acidic subunit and 22-23kD basic subunit of glutelin and the 13kD prolamine could be showed clearly.By this means,we identified some of indica and japonica rice containing higher glutelin content and lower prolamine content.This research result is used as the reference for choosing parents in breeding new conventional rice and hybrid rice containing higher glutelin content.