| 用ELISA测乳汁孕酮进行奶牛的发情检查和早孕诊断的研究 |
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| 引用本文： | 石放雄,吴兰生. 用ELISA测乳汁孕酮进行奶牛的发情检查和早孕诊断的研究[J]. 中国畜牧杂志, 1987, 0(4) |
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| 作者姓名： | 石放雄 吴兰生 |
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| 作者单位： | 浙江农业大学畜牧兽医系(石放雄)，浙江农业大学畜牧兽医系(吴兰生) |
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| 摘 要： | 本试验用ELISA测定了101头奶牛配种期0～2天和配种后18～24天的乳汁孕酮含量,提供了牧场分两阶段测试乳样进行奶牛的发情检查和早孕诊断的可行方案。测定的结果经临床检验核实:发情检查的确诊率为100％;配种后20～22天,妊娠牛和空怀牛的确诊率分别为92.1％和96.9％,可疑率为4.0％,总确诊率为89.9％。
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STUDIES ON THE USE OF ELISA TO DETERMINE THE WHOLE MILK PROGESTERONE LEVEL FOR THE DETECTION OF ESTRUS AND EARLY DIAGNOSIS OF PREGNANCY |
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| Abstract: | Milk from 101 cows during ( day O to day 2 ) and after insemination ( day 18 to day 24 ) were analysed for progesterone concentration by the enzyme-labelled immunosorbent assay ( ELISA ) in order to develop a technique for estrus detection and early pregnancy diagnosis on farm level. The results were verified by clinical inspection. Accuracies of estrus detection were 100% and accuracies of diagnosis for pregnancy and non-pregnancy from day 20 to day 22 after insemination were 92.1% and 96.9% respectively. The overall accuracy was 89.9%. |
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