A microplate reader assay for rapid enzymatic quantification of sugars in potato tubers |
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| Authors: | R. Viola H. V. Davies |
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| Affiliation: | (1) Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, DD2 5DA Dundee, UK |
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| Abstract: | Summary A sensitive, rapid and inexpensive method has been developed for quantifying the glucose, fructose and sucrose content of potato tubers. The method, based on selective enzyme-coupled reaction systems and the reduction of NAD to NADH, uses a microplate reader fitted with a 340 nm filter. In one microtiter plate 96 samples can be analysed for all three sugars in less than two hours. Several plates can be processed in parallel. |
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| Keywords: | glucose fructose sucrose Solanum tuberosum L. |
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