多胺与脱落酸对辣椒子叶再生的影响

 以‘中椒5 号’甜椒和‘湘研10 号’辣椒为试材, 探讨了Spm (精胺) 、Spd (亚精胺) 和ABA(脱落酸) 的不同浓度与不同添加时期对子叶再生的影响。结果表明: 在不定芽分化期和伸长期添加100μmol·L^-1的精胺能显著提高中椒5 号和湘研10 号不定芽的伸长率, 分别较对照提高78%和73%; 在不定芽分化期添加0.3 mg·L^-1的ABA 能促进不定芽分化, 两品种分别较对照提高52.8 %和71.6 %。

Effects of Polyamine and ABA on Regeneration from Cotyledon of Pepper

‘Zhongjiao 5’and‘Xiangyan 10’were used as experimental materials , The effects of different concentration of PA (Spd , Spm) and ABA and added at different stages on regeneration from cotyledon of pepper were studied. The results are following : (1) The rate of adventitious bud elongation is increased obviously by adding 100μmol·L^-1 Spm at both different and elongating stages ; The rate of elongation on ‘Zhongjiao 5’and ‘Xiangyan 10’are increased 78%, 73% respectively compared with control ; (2) The rate of adventitious bud elongation is increased significantly by adding 0.3 mg·L^-1 ABA at different stages. The rate of elongation on ‘Zhongjiao 5’and‘Xiangyan 10’are increased by 52.8 % , 71.6 % respectively over the control .