SD大鼠2型糖尿病模型的建立及相关指标的测定

通过链脲佐菌素(STZ)结合高糖高脂饮食诱导建立2型糖尿病大鼠模型。30只健康雄性SD大鼠随机分为模型组(20只)、空白组(10只)。模型组喂饲高糖高脂饲料4周后,用STZ 30mg/kg一次性左下腹腔注射。检测试验第1周、第4周,注射STZ后第1周、第4周、第8周、第12周空腹体重、血清葡萄糖(BG)、甘油三酯(TG)、胆固醇(TC)、胰岛素(INS)、葡萄糖依赖性促胰岛素释放肽(GIP)、胰岛素样生长因子1(IGF-1)、胰高血糖素样多肽-1(GLP-1)等指标,进行统计分析。处死大鼠,取胰腺进行病理切片检查。动物成模率为75%。试验第4周,模型组TG、TC值明显升高,与空白组比较,P<0.01。注射STZ后第1、4、8、12周,模型组BG、TG、TC值均明显升高,与空白组比较,P<0.01;ISI均明显降低,与空白组比较,P<0.01。注射STZ后第1、4、8周,模型组的GLP-1、IGF-1值明显降低,与空白组比较,P<0.01。注射STZ后第12周,模型组的GLP-1、GIP、IGF-1值明显降低,与空白组比较,P<0.01。试验结果表明,STZ一次性左下腹腔注射结合高糖高脂饮食可成功诱导2型糖尿病大鼠模型。GIP、IGF-1、GLP-1等细胞因子参与了2型糖尿病的病理发生。

Establishment of SD Rat Model of Type 2 Diabetes Mellitus and Detection of Related Indexes

In order to establish a rat model of type 2 diabetes mellitus(T2DM) induced by streptozotocin(STZ) in combination with high-sugar and high-fat diets,30 healthy male SD rats were randomly divided into the T2DM model group(20 rats) and the blank group(10 rats).STZ(30 mg/kg)was injected into rats in the T2DM model group.Rats in the T2DM model group were fed with high-sugar and high-fat diets for 4 weeks.The fasting blood glucose(BG),blood triglyceride(TG),blood cholesterol(TC),body weight,fasting insulin(INS),glucose dependent insulinotropic releasing peptide(GIP),insulin-like growth factor-1(IGF-1),glucagon like peptide-1(GLP-1) were detected in first week,fourth week and after first week,fourth week,eighth week,twelfth week after STZ injection.The sections of pancreas were detected by histopathological examination.The success rate was 75%.Values of TG,TC in T2DM model group increased significantly than those in blank group(P<0.01)after 4 weeks.Values of BG,TG,TC in T2DM model group increased significantly than those in blank group(P<0.01)1,4,8,12 weeks after injection of STZ;ISI decreased significantly than those in blank group,P<0.01;values of GLP-1,IGF-1 in T2DM model group decreased significantly than those in blank group(P<0.01)1,4,8 weeks after injection of STZ;values of GLP-1,IGF-1,GIP in T2DM model group decreased significantly than those in blank group(P<0.01)12 weeks after injection of STZ.The method of inducing T2DM by high-sugar and high-fat diet with STZ injection was effective.GIP,IGF-1,GLP-1 involved in the pathogenesis of type 2 diabetes mellitus.