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猪精液中五种病毒的初步检测
引用本文:张志,刘爽,张燕霞,吴发兴,郑辉,李晓成,黄保续. 猪精液中五种病毒的初步检测[J]. 动物医学进展, 2012, 33(7): 112-115
作者姓名:张志  刘爽  张燕霞  吴发兴  郑辉  李晓成  黄保续
作者单位:中国动物卫生与流行病学中心,山东青岛,266032
基金项目:十一五科技支撑计划课题,广西科技攻关课题
摘    要:为探明猪精液在疫病传播中的作用,应用PCR和RT-PCR技术分别从发病猪场和未发病猪场采集种公猪精液进行猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV-2)和牛病毒性腹泻病毒(BVDV)的检测。结果发现,发病猪场的种公猪精液中CSFV、PRRSV、PRV、PCV-2的感染率分别为30.0%、50.0%、4.0%和30.0%,而未发病猪场的感染率分别为4.0%、20.0%、0.0%和8.0%,这表明精液是多种猪病原传播的良好媒介,提示种公猪对于疫病的发生和传播起着重要作用。

关 键 词:种公猪  精液  病毒测

Detection of Five Viruses in Boar Semen
ZHANG Zhi , LIU Shuang , ZHANG Yan-xia , WU Fa-xing , ZHENG Hui , LI Xiao-cheng , HUANG Bao-xu. Detection of Five Viruses in Boar Semen[J]. Progress In Veterinary Medicine, 2012, 33(7): 112-115
Authors:ZHANG Zhi    LIU Shuang    ZHANG Yan-xia    WU Fa-xing    ZHENG Hui    LI Xiao-cheng    HUANG Bao-xu
Affiliation:(China Animal Health and Epidemiology Center,Qingdao,Shandong,266032,China)
Abstract:To clarify the pathogenicity of boar semen,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Pseudorabies virus(PRV),Porcine circovirus type 2(PCV-2) and Bovine viral diarrhea virus(BVDV) were detected from boar semens collected from clinical and healthy pig farms.The detections showed that infection rate of CSFV,PRRSV,PRV,PCV-2 existed in clinical pig farms with 30.0%,50.0%,4.0% and 30.0%,while a lower infection accounted for 4.0%,20.0%,0.0% and 8.0% in healthy pig farms,respectively.It indicated strongly that the boar played a role in the occurrence and spread of diseases.
Keywords:breeding boar  semen  virus detection
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