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秦川牛血红素提取工艺的研究
引用本文:马志科,昝林森,马丽.秦川牛血红素提取工艺的研究[J].动物医学进展,2012,33(6):84-86.
作者姓名:马志科  昝林森  马丽
作者单位:西北农林科技大学动物科技学院,陕西杨凌,712100
基金项目:农业部公益性行业科研专项
摘    要:以秦川牛血为原料提取血红素,选择影响提取的主要因素——氯仿加入量、酸性丙酮加入量、pH分别作为单因素进行梯度试验,确定其合适范围,进一步通过正交试验选取最佳提取条件。结果表明,提取血红素的最佳工艺为氯仿加入量为血溶体积的5倍,酸性丙酮加入量为血球蛋白滤液的5倍,调节pH4~5。

关 键 词:秦川牛  血红素  提取工艺

Study on Technology of Extracting Heme from Qinchuan Cattle Blood
MA Zhi-ke , ZAN Lin-sen , MA Li.Study on Technology of Extracting Heme from Qinchuan Cattle Blood[J].Progress In Veterinary Medicine,2012,33(6):84-86.
Authors:MA Zhi-ke  ZAN Lin-sen  MA Li
Institution:(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,712100,China)
Abstract:Heme extracted from Qinchuan cattle blood was studied.The main factors,namely chloroform added,acid acetone added,pH were chosen to carry on the gradient test,as single factor respectively,to make the range of each single factor.The orthogonal trial of L9(33) was used to select the suitabl combination conditions of extraction.The results showed that the optimum condition for the extraction of heme were: chloroform and blood volume at a ration of 5∶1(V∶V),acid acetone and hemoglobin solution at a ration of 5∶1(V∶V) and pH 4-5.
Keywords:Qinchuan cattle  heme  extraction technology
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