| 猪博卡病毒实时荧光定量PCR检测方法的建立 |
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| 引用本文： | 邓波,刘佩红,周锦萍,王建,刘健,鞠厚斌,葛菲菲,崔立,华修国.猪博卡病毒实时荧光定量PCR检测方法的建立[J].动物医学进展,2012(9):70-74. |
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| 作者姓名： | 邓波 刘佩红 周锦萍 王建 刘健 鞠厚斌 葛菲菲 崔立 华修国 |
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| 作者单位： | 1. 上海市动物疫病预防控制中心,上海201103
2. 上海交通大学上海市兽医生物技术重点实验室,上海200240 |
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| 摘 要： | 根据GenBank公布的猪博卡病毒(PBoV)序列,通过VP1/2基因设计引物和Taq Man探针建立实时荧光定量PCR检测方法。建立的方法与PPV、PRRSV及PCV均无交叉反应,具有较高特异性,在107 copies/mL～101 copies/mL模板范围内具有良好的线性关系,所制作的标准曲线相关系数为0.997,最低可检测到101copies/mL的阳性质粒。说明所建立的PBoV实时荧光定量PCR检测方法具有灵敏度高、特异性好和精确性高等优点。
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| 关 键 词： | 猪博卡病毒 实时荧光定量PCR Taq Man探针 |
Development of a Real-time PCR for Detection of Porcine Bocavirus |
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| DENG Bo,LIU Pei-hong,ZHOU Jing-ping,WANG Jian,LIU Jian,JU Hou-bin,GE Fei-fei,CUI Li,HUA Xiu-guo.Development of a Real-time PCR for Detection of Porcine Bocavirus[J].Progress In Veterinary Medicine,2012(9):70-74. |
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| Authors: | DENG Bo LIU Pei-hong ZHOU Jing-ping WANG Jian LIU Jian JU Hou-bin GE Fei-fei CUI Li HUA Xiu-guo |
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| Institution: | 1.Shanghai Center for Animal Disease Control,Shanghai,201103,China;2.Department of Agriculture& Biology,Shanghai Jiao tong University,Shanghai,200240,China) |
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| Abstract: | A TaqMan based real-time PCR was developed for the specific detection of porcine bocavirus virus(PBoV).The specific primers and probes were designed according to the VP1 / 2genes of PBoV in GenBank.The result showed that the specificity of this assay was high without any cross-reaction with PPV,PRRSV and PCV.A good linear correlation was demonstrated in the standard curve for the real-time PCR assay within the range of 10 1 to 10 7 copies / mL,with a correlation coefficient of 0.997.The detection limit the real-time PCR assay was 10 1 copies / mL of positive plasmids,indicating agood sensitivity.This real-time PCR based on TaqMan probe technology is a high sensitive and specific for detecting PBoV. |
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| Keywords: | Porcine bocavirus real-time PCR TaqMan probe |
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