辣椒β–酮脂酰辅酶A合酶基因家族的鉴定与表达分析

为深入了解β–酮脂酰辅酶A合酶(β-ketoacyl-CoAsynthase,KCS)基因家族在辣椒基因组中的特征,利用生物信息学方法鉴定所有KCS基因家族成员,对基因染色体定位、系统发育关系、基因结构、蛋白保守基序和组织表达模式等进行分析,并通过qRT-PCR方法检测基因在高温、低温和NaCl胁迫下的响应。从辣椒基因组中共鉴定出22个KCS家族基因,其不均匀地分布在辣椒的9条染色体上;系统发育分析表明辣椒KCS基因家族可分为4组,每组含有不同数量的基因;该家族成员含有0~3个内含子,保守基序有6~14个;KCS家族基因具有不同的表达模式;高温、低温和NaCl处理可以抑制或激活该家族成员的表达。

Identification and Expression Analysis of the KCS Gene Family in Pepper

In order to understand the characteristics of theβ-ketoacyl-CoA synthase(KCS)gene family in the Capsicum annuum genome deeply,all KCS gene family members were identified by using the bioinformatics methods,hereafter,the genes chromosomal location,phylogenetic relationship,gene structure,protein conserved motifs,tissue expression pattern and response to high temperature,low temperature and NaCl stress were analyzed.The results showed that a total of 22 KCS genes were identified and distributed on 9 chromosomes.Phylogenetic analysis indicated that the CaKCS were divided into 4 groups,and each of them included different numbers of genes which contained 0–3 introns and 6–14 conserved motifs.Each gene in the KCS family has different expression patterns,and the CaKCS could be repressed or activated by high temperature,low temperature and NaCl treatment.