棉花秸秆浸提液对小麦根系生长的影响

目的]研究棉花秸秆浸提液对小麦根系生长的影响,明确棉花秸秆对小麦根系的化感效应。方法]以小麦品种扬麦13为材料,设置不同棉花秸秆浸提液水平(0%、1.0%、2.0%、4.0%、8.0%和10.0%),研究浸提液对小麦根系形态指标和生理活性的影响。结果]棉花秸秆浸提液抑制了小麦根系的伸长,增加了根系平均直径,导致根表面积和根体积降低;浸提液处理诱导了根系活性氧含量升高,导致根系膜脂过氧化程度加剧,进而降低了根系活力,抑制了根系吸水和生物量形成;随秸秆浸提液升高,浸提液中总酚酸含量持续升高,总酚酸含量与根系形态建成和根系生理活性密切相关。结论]棉花秸秆浸提液通过影响根系活性氧变化限制了根系生长发育并降低了生理活性,浸提液中总酚酸含量是主要限制因子之一。

Effect of Cotton Straw Extract on Wheat Root Growth

Objective] The aim was to study the effects of cotton straw extract on wheat root growth and define the al elopathic effect of cotton straw on wheat root. Method] A sand culture experiment was conducted under different cotton straw ex-tract levels （0%, 1.0%, 2.0%, 4.0%, 8.0% and 10.0%）, and the morphology and physiological activity of wheat （Yangmai 13） root were studied. Result] The cotton straw extract inhibited the elongation of wheat roots, but increased the root diame-ter, resulting in the decreased root surface area and volume; The extract treatments induced the increase of root reactive oxygen species （ROS） level, which led to an increased root membrane lipid peroxidation, thereby reducing the root vigor and in-hibiting the root water uptake and formation of biomass; With the increase of con-centration of cotton straw extract, the total phenolic acid content in the extract in-creased continuously, and the total phenolic acid content was closely related to the morphogenesis and physiological activity of wheat root. Conclusion] The cotton straw extract inhibited the morphogenesis and physiological activity of wheat root through affecting the root ROS level, and the total phenolic content was one of the main limiting factors.