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| 影响美国速生生菜愈伤组织诱导因素的研究 | |
| 引用本文： | 高晔华,朱世,李金凤,郭朋伟,吴荣哲.影响美国速生生菜愈伤组织诱导因素的研究[J].延边大学农学学报,2011,33(4):290-293. |
| 作者姓名： | 高晔华 朱世 李金凤 郭朋伟 吴荣哲 |
| 作者单位： | 1. 延边大学农学院,吉林延吉,133002 2. 两江镇农业技术推广站,吉林安图,133600 3. 延边职业技术学院,吉林延吉,133000 |
| 摘 要： | 以美国速生生莱为试材，探索不同浓度的2，4-D对生菜根、茎、叶不同外植体诱导愈伤组织的影响，进一步探索不同浓度2，4-D、蔗糖和不同光条件对生菜叶片愈伤组织诱导的影响．结果表明：生菜根、茎、叶在2，4-D浓度为1mg／L时外植体叶诱导的愈伤组织各项指标均较好；0．6mg／L2，4-D、20g／L蔗糖、光培养处理时对提高叶片愈伤组织的鲜物重及干物重指标的效果最好，诱导率可达到89．8％． |
| 关 键 词： | 生菜 愈伤组织 蔗糖浓度 2，4-D浓度 光暗培养 |
Study of condition on induction of callus in Lactuca sativa. L. |
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| GAO Ye-hua,ZHU Shi,LI Jin-feng,GUO Peng-wei,WU Rong-zhe.Study of condition on induction of callus in Lactuca sativa. L.[J].Journal of Agricultural Science Yanbian University,2011,33(4):290-293. | |
| Authors: | GAO Ye-hua ZHU Shi LI Jin-feng GUO Peng-wei WU Rong-zhe |
| Institution: | 1*) (1.Agricultural College of Yanbian University,Yanji Jilin 133002,China; 2.Liangjiang Town Agricultural Technology Extension Station,Antu Jilin 133600,China; 3.Yanbian Vocational and Technical College,Yanji Jilin 133000,China) |
| Abstract: | Make the leaves callus of Grand Rapids TBR （Lactuca sativa var. L） as the material,explore different concentrations of 2,4-D on the impacl： of vegetable root, stem and leaf callus, and explore different concentrations of 2,4-D on induction of callus in lettuce. The results showed that the root, stem and leaf of lettuce callus growth best in the concentration of 2,4-D is 1 mg/L,and the influence of 0.6 mg/L 2,4-D,20 g/L sucrose medium and light culture are the best to improve callus. The induction rate reached 89.89%. |
| Keywords: | lettuce callus 2 4-D concentration sucrose concentration light and dark cultures |
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