| 体外培养条件下维生素E对瘤胃培养液中共轭亚油酸含量的影响 |
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| 引用本文： | 王芳,刘飞,杜鹏,侯俊财.体外培养条件下维生素E对瘤胃培养液中共轭亚油酸含量的影响[J].东北农业大学学报,2011,42(11):45-50. |
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| 作者姓名： | 王芳 刘飞 杜鹏 侯俊财 |
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| 作者单位： | 1. 乳品科学教育部重点实验室,哈尔滨150030;东北农业大学食品学院,哈尔滨150030
2. 乳品科学教育部重点实验室,哈尔滨,150030 |
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| 基金项目： | 黑龙江省青年科学基金(QC08C26); 东北农业大学博士启动基金 |
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| 摘 要： | 采用批次培养法研究了体外条件下高油日粮中添加维生素E对山羊瘤胃培养液中共轭亚油酸累积规律的影响.选择6只带有永久胃瘘管的山羊制备出瘤胃培养液进行体外培养24h,维生素E添加量分别为0、0.5、1.0和2.0 ng·瓶-1,每组设三个重复,测量其发酵液中共轭亚油酸含量变化.结果显示,在培养8h和16h时,VE各处理组的顺...
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| 关 键 词： | 瘤胃培养液 维生素E 共轭亚油酸 |
Effect of Vitamin E on concentration of conjugated linoleic acid in vitro |
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| WANG Fang,LIU Fei,DU Peng,HOU Juncai.Effect of Vitamin E on concentration of conjugated linoleic acid in vitro[J].Journal of Northeast Agricultural University,2011,42(11):45-50. |
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| Authors: | WANG Fang LIU Fei DU Peng HOU Juncai |
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| Institution: | WANG Fang1,2,LIU Fei1,DU Peng1,HOU Juncai1,2(1.Key Laboratory of Dairy Science of Ministry of Education,Harbin 150030,China,2.Food College,Northeast Agricultural University,China) |
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| Abstract: | The study was conducted to investigate the effect of addition of vitamin E in high soybean oil diet on concentration of conjugated linoleic acid of mature goat in vitro by batch culture.The ruminal culture fluid collected from 6 fistulated mature goats was incubated for 24 hours.The addition levels of the vitamin E were 0(control group),0.5,1.0 and 2.0 mg·flask-1,respectively.The results indicated that addition of vitamin E in diet of high soybean oil could influence the content of conjugated linoleic acid.At the incubation of 8 h and 16 h,the contents of cis-9 and trans-11 CLA in VE supplementation treatments were significantly lower than those of control group(P<0.05),which of VE3 treatments were lower than that of the control group,0.97 mg·g-1 DM and 2.34 mg·g-1 DM,respectively.But after the incubation of 24 hours,the contents of cis-9 and trans-11 CLA of VE supplementation treatments were significantly higher than those of the control group(P<0.05).After the incubation of 2 hours,the contents of trans-10 and cis-12 CLA in VE supplementation treatments were significantly lower than those of control group(P<0.05),which in VE3 group was lower(0.41 mg·g-1 DM) those that of the control group.The contents of cis-9 and trans-11 CLA + trans-11 C18:1 of VE2 and VE3 weren't affected,comparing with control group all the time.Concentration of conjugated linoleic acid was affected by VE supplementation. |
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| Keywords: | crumen culture fluid vitamin E conjugated linoleic acid |
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