| 黑曲霉高产纤维素酶菌株的高通量筛选 |
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| 引用本文： | 李兆周,王学红,陈化靓,梁剑平.黑曲霉高产纤维素酶菌株的高通量筛选[J].甘肃农业大学学报,2011,46(5):151-156. |
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| 作者姓名： | 李兆周 王学红 陈化靓 梁剑平 |
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| 作者单位： | 1. 中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省/中国农业科学院新兽药工程重点实验室,甘肃兰州730050
2. 兰州理工大学生命科学与工程学院,甘肃兰州,730050 |
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| 基金项目： | 中国科学院“百人计划”(O861010ZY0) |
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| 摘 要： | 以黑曲霉GSICC 60108为出发菌株,经紫外和电子束诱变处理后,采用纤维素-刚果红平板初筛和新建立的高通量微孔板法进行复筛以获得高产纤维素酶菌株.结果表明:试验得到1株稳定高产的纤维素酶菌株黑曲霉EBR-106；所建立的复筛方法的标准曲线在葡萄糖质量浓度为0～10 mg/mL的范围内线性良好(r=0.999 75)...
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| 关 键 词： | 黑曲霉 纤维素酶 纤维素-刚果红平板 微孔板筛选法 |
High-throughput screening of Aspergillus niger strains with high production of cellulase |
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| LI Zhao-zhou,WANG Xue-hong,CHEN Hua-liang,LIANG Jian-ping.High-throughput screening of Aspergillus niger strains with high production of cellulase[J].Journal of Gansu Agricultural University,2011,46(5):151-156. |
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| Authors: | LI Zhao-zhou WANG Xue-hong CHEN Hua-liang LIANG Jian-ping |
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| Institution: | LI Zhao-zhou1,WANG Xue-hong1,CHEN Hua-liang2,LIANG Jian-ping1(1.Key Laboratory of New Animal Drug Discovery of Agricultural Ministry,Key Laboratory of New Animal Drug Projects of Gansu Province/CAAS,Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China,2.College of Life Science and Engineering,Lanzhou University of Technology,China) |
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| Abstract: | To obtain a high cellulase-producing strain,the parental strain Aspergillus niger GSICC 60108 was subjected to mutation involving irradiation with ultraviolet light and subsequently with electron beam.The results showed that after the primary screening by cellulose Congo-Red medium and secondary high-throughput screening with newly development of microplate-based filter paper assay,the stable mutant Aspergillus niger EBR-106 was obtained.The standard curve of secondary screening method performed a good line... |
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| Keywords: | Aspergillus niger cellulase Congo-red plate screening with microplate-based filter paper assay |
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