| 进境玉米种子中玉米褪绿斑驳病毒的检测鉴定 |
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| 引用本文： | 刘洪义,刘忠梅,张金兰,张洪祥. 进境玉米种子中玉米褪绿斑驳病毒的检测鉴定[J]. 东北农业大学学报, 2011, 42(10): 36-40 |
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| 作者姓名： | 刘洪义 刘忠梅 张金兰 张洪祥 |
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| 作者单位： | 黑龙江出入境检验检疫局检验检疫技术中心,哈尔滨,150001;黑龙江出入境检验检疫局邮局办事处,哈尔滨,150001 |
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| 基金项目： | 国家质检总局资助项目(2009IK255) |
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| 摘 要： | 2011年3月,黑龙江出入境检验检疫局技术中心植检室先后接收两批进境的玉米种子,标明来自德国,报验号分别为:11201100021、11201100022,要求检测玉米褪绿斑驳病毒等检疫性有害生物.分别选取300粒种子进行两组室内盆栽试植试验,11201100021组观察到两株具有典型褪绿斑驳症状的病苗；1120110...
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| 关 键 词： | 玉米种子 玉米褪绿斑驳病毒 外壳蛋白基因 序列分析 ELISA检测 |
Detection and identification of Maize chlorotic mottle virus from imported maize seeds |
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| LIU Hongyi,LIU Zhongmei,ZHANG Jinlan,ZHANG Hongxiang. Detection and identification of Maize chlorotic mottle virus from imported maize seeds[J]. Journal of Northeast Agricultural University, 2011, 42(10): 36-40 |
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| Authors: | LIU Hongyi LIU Zhongmei ZHANG Jinlan ZHANG Hongxiang |
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| Affiliation: | LIU Hongyi1,LIU Zhongmei1,ZHANG Jinlan2,ZHANG Hongxiang1(1.Technical Center of Inspection and Quarantine,Heilongjiang Entry-Exit Inspection and Quarantine Bureau,Harbin 150001,China,2.Post Office Branch,China) |
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| Abstract: | Two lots of maize seeds from Germany were sent to Technical Center of Inspection and Quarantine of Heilongjiang Entry-Exit Inspection and Quarantine Bureau to test Maize chlorotic mottle virus (MCMV) and other quarantine pests in March 2011,and marked No.11201100021 and No.11201100022,respectively.Two lots of imported maize seeds were planted in a quarantine labora-tory,respectively.Two seedlings of No.11201100021 lot with chlorotic mottle symptoms were observed.One seedling of No.11201100022 lot with chlor... |
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| Keywords: | maize seeds Maize chlorotic mottle virus (MCMV) coat protein gene sequence analysis ELISA test |
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