青海蚕豆种质资源AFLP多样性分析和核心资源构建

采用AFLP分子标记技术分析了青海蚕豆种质资源的遗传多样性.结果表明:地方品种资源、国外种质和选育品种的有效等位变异分别为(1.065 5±0.126 3)、(1.063 4±0.143 0)、(1.065 9±0.165 1)；Shannon's信息指数分别为(0.095 4±0.144 9)、(0.084 3±0....

Diversity analysis on germplasm resources by AFLP and core resources construction of Vicia faba in Qinghai

The diversity on germplasm of Faba bean(Vicia faba L.) in Qinghai was analyzed by AFLP.The results showed that the effective number alleles of landraces,foreign germplasms and bred varieties were(1.065 5±0.126 3),(1.063 4±0.143 0),and(1.065 9±0.165 1),respectively.The Shannon′s information index was(0.095 4±0.144 9),(0.084 3±0.151 8),(0.074 2±0.167 9),respectively.The radio of effective allele of landrace was the lowest(73.73%),while its ploy-radio was the highest(44.51%).The radio of effective allele of bred varieties was the highest(90.39%),while its ploy-radio was the lowest(17.92%).The genetic distance was relatively large in landraces and small in foreign germplasms.Genetic structure analysis revealed that 149 Faba bean germplasm resources in Qinghai should be divided into 7 clusters.40 core resources were constructed,containing more than 80% gene information of the 149 resources,with higher total gene diversity and Nei′s gene diversity,which could be used as good parent materials for cross breeding.