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| BtR05的鉴定及其对朱砂叶螨的毒力测定 | |
| 引用本文： | 赵晓燕,李纪顺,周红姿,张新建,王贻莲,魏艳丽,杨合同.BtR05的鉴定及其对朱砂叶螨的毒力测定[J].福建农林大学学报(自然科学版),2011,40(4):341-345. |
| 作者姓名： | 赵晓燕 李纪顺 周红姿 张新建 王贻莲 魏艳丽 杨合同 |
| 作者单位： | 1. 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室 2. 山东省科学院生物研究所,山东济南,250014 3. 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室;山东省科学院生物研究所,山东济南250014 |
| 基金项目： | 山东省科技攻关项目(2010GSF10206、2006GG2209002) |
| 摘 要： | 对山东省应用微生物重点实验室分离保存的苏云金芽孢杆菌BtR05进行了16S rDNA序列分析,结合菌落形态、生理生化特征研究,将其鉴定为苏云金芽孢杆菌属.通过与已知的苏云金芽孢杆菌29个亚种间的生理生化特征进行比较,鉴定BtR05为苏云金芽孢杆菌达姆斯特亚种(Bacillus thuringiensis subsp.d... |
| 关 键 词： | 苏云金芽孢杆菌BtR05 16S rDNA序列 生理生化特征 朱砂叶螨 校正死亡率 |
Identification of BtR05 and toxicity tests to Tetranychus cinnabarinus(Boisduval) |
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| ZHAO Xiao-yan,LI Ji-shun,ZHOU Hong-zi,ZHANG Xin-jian,WANG Yi-lian,WEI Yan-li,YANG He-tong.Identification of BtR05 and toxicity tests to Tetranychus cinnabarinus(Boisduval)[J].Journal of Fujian Agricultural and Forestry University,2011,40(4):341-345. | |
| Authors: | ZHAO Xiao-yan LI Ji-shun ZHOU Hong-zi ZHANG Xin-jian WANG Yi-lian WEI Yan-li YANG He-tong |
| Institution: | ZHAO Xiao-yan1,LI Ji-shun1,ZHOU Hong-zi1,ZHANG Xin-jian1,WANG Yi-lian1,WEI Yan-li2,YANG He-tong1,2(1.Shandong Provincial Key Laboratory of Applied Microbiology,China-Japan Friendship Biotechnology Research Center,2.Biology Research Institute,Shandong Academy of Sciences,Jinan,Shandong 250014,China) |
| Abstract: | In this paper,the strain BtR05 was identified as Bacillus thuringiensis based on morphological,physiological and biochemical characteristics,combined with analysis of 16S rDNA sequences.Through comparing the physiological and biochemical characteristics of BtR05 with other 29 known B.thuringiensis subspecies,it was identified as B.thuringiensis subsp.darmstadiensis.The toxicities of BtR05 fermentation broth to the larvae,nymphae and adult of Tetranychus cinnabarinus were tested.The results showed that its t... |
| Keywords: | Bacillus thuringiensis BtR05 16S rDNA sequence physiological and biochemical characteristics Tetranychus cinnabarinus corrected mortality |
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