| 烟草谷氧还蛋白基因NbGRX1的克隆与表达特性分析 |
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| 引用本文： | 郭玉双,张建华,张吉顺,史跃伟,陈静.烟草谷氧还蛋白基因NbGRX1的克隆与表达特性分析[J].东北农业大学学报,2011,42(10):47-52. |
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| 作者姓名： | 郭玉双 张建华 张吉顺 史跃伟 陈静 |
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| 作者单位： | 1. 贵州省烟草科学研究所,贵阳,550081
2. 浙江大学环境与资源学院环境工程系,杭州,310058 |
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| 基金项目： | 贵州省科学基金项目(黔科合J字[2011]2399号) |
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| 摘 要： | 采用同源克隆及cDNA末端快速扩增法(RACE),从烟草中克隆到1个谷氧还蛋白基因(Grx),命名为NbGRX1.该基因全长1 021 bp,编码298个氨基酸,具有典型的Grx基因结构域.Real-time RT-PCR分析表明,该基因能在烟草的根、茎、叶中表达,并且受低温、干旱和高盐诱导.
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| 关 键 词： | 本氏烟 谷氧还蛋白基因 荧光定量PCR |
Cloning and expression pattern analysis a novel NbGRX1 gene from Nicotiana benthamiana |
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| GUO Yushuang,ZHANG Jianhua,ZHANG Jishun,SHI Yuewei,CHEN Jing.Cloning and expression pattern analysis a novel NbGRX1 gene from Nicotiana benthamiana[J].Journal of Northeast Agricultural University,2011,42(10):47-52. |
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| Authors: | GUO Yushuang ZHANG Jianhua ZHANG Jishun SHI Yuewei CHEN Jing |
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| Institution: | GUO Yushuang1,ZHANG Jianhua2,ZHANG Jishun1,SHI Yuewei1,CHEN Jing1(1.Guizhou Tobacco Research Institute,Guiyang 550081,China,2.Department of Environmental Engineering,College of Environment and Resources Science,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | A cDNA encoding a protein containing the consensus Grx family domain with a CGFS active site was isolated using homologue cloning and RACE methods.Quantitative real-time RT-PCR (qRT-PCR) analysis revealed that NbGRX1 is expressed ubiquitously in tobacco including leaf,root,and stem,and can be induced by low temperature,drought and salt stresses. |
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| Keywords: | Nicotiana benthamiana glutaredoxin gene quantitative real-time RT-PCR |
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