| 超声波-双水相协同提取海带多糖工艺的研究 |
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| 引用本文： | 刘宝亮,曹桂萍,张金涛,张梅.超声波-双水相协同提取海带多糖工艺的研究[J].安徽农业科学,2010,38(31):17445-17447. |
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| 作者姓名： | 刘宝亮 曹桂萍 张金涛 张梅 |
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| 作者单位： | 常州工学院理学院,江苏常州,213002;常州工学院理学院,江苏常州,213002;常州工学院理学院,江苏常州,213002;常州工学院理学院,江苏常州,213002 |
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| 基金项目： | 江苏省高校自然科学指导项目(09KJD150010) |
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| 摘 要： | 目的]优化超声波-双水相协同提取海带多糖的工艺。方法]采用丙醇-硫酸铵双水相体系与超声耦合对海带多糖进行提取．苯酚-硫酸法测定多糖含量；采用单因素试验优化海带多糖的提取工艺。结果]海带多糖提取的最佳工艺为：浸提时间为2h，超声提取时间为30min．提取温度为60℃，料液比为0．019，提取液pH值为5，在此条件下海带多糖的提取率为11．45％。结论]试验建立了海带多糖提取的最佳工艺，为海带多糖的高效提取奠定了基础。
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| 关 键 词： | 超声波-双水相协同提取 海带 多糖 提取工艺 |
Study on Extraction Process of Polysaccharide from Laminaria japonica Aresch.by Ultrasonic-Aqueous Two Phase System Assisted Extraction |
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| LIU Bao-liang et al.Study on Extraction Process of Polysaccharide from Laminaria japonica Aresch.by Ultrasonic-Aqueous Two Phase System Assisted Extraction[J].Journal of Anhui Agricultural Sciences,2010,38(31):17445-17447. |
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| Authors: | LIU Bao-liang |
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| Institution: | LIU Bao-liang et al(School of Science,Changzhou Institute of Technology,Changzhou,Jiangsu 213002) |
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| Abstract: | Objective] The aim was to optimize the extraction process of polysaccharide from Laminaria japonica Aresch.by ultrasonic-aqueous two phase system assisted extraction.Method]The acetone-ammonium sulfate system coupling with ultrasonic extraction was used to extract the polysaccharide from Laminaria japonica Aresch.,and the content of polysaccharide was determined with phenol-sulphuric acid.The single factor test was used to optimize the extraction process of polysaccharide.Result]The optimum extraction co... |
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| Keywords: | Ultrasonic-aqueous two phase system assisted extraction Laminaria japonica Aresch Polysaccharide Extraction process |
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