| 利用SSR标记技术构建黄瓜遗传连锁图谱 |
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| 引用本文： | 王军辉,崔兴华,张桂华,杜胜利,张显.利用SSR标记技术构建黄瓜遗传连锁图谱[J].北方园艺,2010(22):115-118. |
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| 作者姓名： | 王军辉 崔兴华 张桂华 杜胜利 张显 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨凌,712100;天津科润黄瓜研究所,天津,300192 |
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| 基金项目： | 天津市应用基础研究计划资助项目，"十一五"国家"863"计划资助项目 |
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| 摘 要： | 利用抗黄瓜花叶病毒病(CMV)的黄瓜材料F-3和感CMV的黄瓜材料HZL04-1为亲本,构建包含190个F2后代的遗传作图群体。结果表明:采用SSR、EST-SSR和SCAR 3种分子标记技术对该群体进行遗传连锁分析,获得1个包含170个SSR标记,6个EST-SSR标记,3个SCAR标记的黄瓜遗传连锁图谱,该图谱涉及7个连锁群,总长度为862.886 cM,平均图距为4.79 cM。该图谱的构建为黄瓜抗CMV的QTL定位奠定了基础,并确定了连锁群与染色体之间的对应关系。
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| 关 键 词： | SSR EST-SSR SCAR 遗传连锁图谱 黄瓜 |
Construction of Genetic Linkage Map of Cucumber by SSR Technology |
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| WANG Jun-hui,CUI Xing-hua,ZHANG Gui-hua,DU Sheng-li,ZHANG Xian.Construction of Genetic Linkage Map of Cucumber by SSR Technology[J].Northern Horticulture,2010(22):115-118. |
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| Authors: | WANG Jun-hui CUI Xing-hua ZHANG Gui-hua DU Sheng-li ZHANG Xian |
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| Institution: | WANG Jun-hui1,CUI Xing-hua2,ZHANG Gui-hua2,DU Sheng-li2,ZHANG Xian1(1.College of Horticulture,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi 712100,2.Tianjin Kernel Cucumber Research Institute,Tianjin 300192) |
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| Abstract: | With a CMV-resistant line(F-3) and a CMV-susceptible line(HZL04-1) as parents,an F2 genetic mapping population containing 190 F2 progenies was constructed.The results showed that 1 cucumber genetic linkage map containing 7 linkage groups was constructed using 170 SSR,6 EST-SSR,and 3 SCAR markers,and the map covered 862.886 cM with an average marker interval of 4.79 cM.It was the basis of QTL locating of CMV resistant gene.The correspondence between the linkage groups and chromosomes was determined by compar... |
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| Keywords: | SSR EST-SSR SCAR genetic linkage map cucumber |
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