| 小红参愈伤组织的诱导及分化 |
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| 引用本文： | 罗春梅,邱璐,杨清辉,萧凤回,刘爱民. 小红参愈伤组织的诱导及分化[J]. 华中农业大学学报, 2006, 25(2): 182-185 |
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| 作者姓名： | 罗春梅 邱璐 杨清辉 萧凤回 刘爱民 |
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| 作者单位： | 云南省楚雄农业学校,楚雄,675000;云南省楚雄师范学院,楚雄,675000;云南农业大学农学院,昆明,650201 |
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| 基金项目： | 云南省科技厅云南省中药现代化科技产业专项基金 |
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| 摘 要： | 通过不同外植体、基本培养基、暗培养、激素对小红参愈伤组织诱导和分化的试验研究,结果表明诱导小红参愈伤组织产生的最佳条件为:以真叶和顶芽(或侧芽)为外植体,在MS BA 0.2 mg/L NAA 3.0mg/L培养基上,进行暗培养,愈伤诱导率达95%。芽形成的愈伤组织组织在MS BA 5.0 NAA 1.5~2.0中均能分化出幼苗,分化率达100%,但叶形成的愈伤组织未能分化出苗。
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| 关 键 词： | 小红参 组织培养 愈伤组织 不定芽 |
| 文章编号： | 1000-2421（2006）02-0182-04 |
| 收稿时间： | 2005-11-06 |
| 修稿时间： | 2005-11-06 |
Callus Induction and Bud Formation of Rubia yunnanensis (Franch.) Diels |
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| LUO Chun-mei,QIU Lu,YANG Qing-hui,XIAO Feng-hui,LIU Ai-min. Callus Induction and Bud Formation of Rubia yunnanensis (Franch.) Diels[J]. Journal of Huazhong Agricultural University, 2006, 25(2): 182-185 |
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| Authors: | LUO Chun-mei QIU Lu YANG Qing-hui XIAO Feng-hui LIU Ai-min |
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| Affiliation: | (1. Chuxiong Agricultural School, Chuxiong, Yunnan 675000, China ; 2. Chuxiong Normal University, Chuxiong Yunnan 675000, China ; 3. Yunnan Agricultural University, Kunming , Yunnan 650201, China |
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| Abstract: | The effect of different plantlet,basic medium,dark culture,hormone combination and densities on the formation and differentiation of callus ofEucalyptus smithiiL was studied.It was found that callus form well in the dark using true leaf and bud as plantlet,MS BA 0.2 mg/L NAA 3.0 mg/L as basic medium with the forming rate of callus up to 95%.The callus from the bud can differenti-ate to form bud using MS BA 0.5 NAA 0.5 as medium with the rate up to 100%.But the callus from the leaf can not differentiate to form adventitious bud. |
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| Keywords: | Eucalyptus smithiiL. tissue culture callus adventitious bud |
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