人工老化对老芒麦醇溶蛋白遗传完整性的影响

以4份老芒麦种质为试验材料,对其人工加速老化后种子活力及其醇溶蛋白的遗传完整性进行了分析。结果表明,随着老化时间的延长,老芒麦种子的发芽率、发芽势、发芽指数和活力指数逐渐降低,其中供试材料的发芽势、活力指数均随发芽率的下降与对照呈极显著差异。与未老化的种子相比,人工老化后种子醇溶蛋白的遗传完整性发生了明显变化。在发芽率降低为55%及以下,育成品种川草1号、农牧由最初的2、3种谱带类型减少为仅表现1种谱带类型,而2份野生材料较对照分别丢失了2~3种谱带类型。老芒麦醇溶蛋白谱带的遗传多样性指数分析结果也显示,当发芽率低于55%时,4份材料的等位基因数均与对照间呈显著差异。因此,建议60%~70%作为老芒麦繁殖更新的参考发芽率标准,最低临界值为55%。

Effects of Artificial Aging on Genetic Integrity of Elymus sibiricus L.

The effects of artificial aging on seed vigor and genetic integrity of gliadin in four Elymus sibiricus L. accessions were studied. The results showed that the germination rate, germination potential, germinating index and vigor index of the Elymus sibiricus L. seeds decreased gradually with the aging time prolonging, the germination potential and vigor index of 4 materials were highly significantly different from CK with the decrease of germination rate among them. The genetic integrity of gliadin also changed obviously compared to CK. Two and three types of gliadin spectrum of varieties Elymus sibiricus L. cv. Chuancao No.1 and Elymus sibiricus L. cv. Nongmu were reduced to only 1 type while the types of gliadin spectrum of 2 wild accessions decreased most obviously than CK when the germination rate reduced to about 55%. The genetic diversity index analysis on gliadin spectrum of Elymus sibiricus L. also showed the allele number of 4 materials was significantly different from that of the control when the germination rate was less than 55%. Therefore, it is recommended that 60%~70% as the reference standard of germination rate for renewingElymus sibiricus L. by reproduction and the lowest critical value is 55%.