HPLC法测定复方多聚甲醛粉中水杨酸和苯甲酸

建立了HPLC法测定复方多聚甲醛粉中水杨酸和苯甲酸的方法。采用AgilentZORBAXEclipseXDB-C18色谱柱（4．6mm×250mm，5μm），以0．05mol／L磷酸二氢钾溶液（pH6．0）-甲醇（90：10）为流动相，流速1．0mL／min，检测波长230nm，柱温40cC，进样量10μL，以外标法定量。水杨酸和苯甲酸在1．0～50μg／mL范围内均线性关系良好，相关系数r均为0．9999，平均回收率分别为99．06％～99．69％（RSD=0．09％～0．63％）和99．12％～99．73％（RSD=0．16％～0．48％），n=3。本方法简便、准确，可快速测定复方多聚甲醛粉中水杨酸和苯甲酸的含量，为产品质量控制提供了依据。

Determination of Salicylic acid and Benzoic acid in Compound Paraformaldehyde Powder by HPLC

To establish a HPLC method for determination of salicylic acid and benzoic acid in compound paraformaldehyde powder,Agilent ZORBAX Eclipse XDB-C18 column （4.6 mm×250 mm, 5μm）was used with the mobile phase of a mixture of methanol and 0.05 mol/L KH2P04（ 10 ： 90 ） （ adjusted to pH 6.0 with H3P04） as mobile phase ;the flow rate was 1.0 mL/min; the column temperture was 40 ℃ ; the detection wavelength was 230 nm. The calibration curves of salicylic acid and benzoic acid was linear relationship in the range of 1.0- 50 μg/mL（r=0.9999）. The average recoveries was 99.06%-99.69% （RSD= 0.09%-0.63%） and 99.12% 99.73% （ RSD = 0.16% - 0.48% ） respectively, n = 3. The established method was simple, quick and accurate for the simultaneous separation and determination of salicylic acid and benzoic acid in compound paraformaldehyde powder.