杨树溃疡病菌致病性分化与酯酶同功酶关系的研究

对北京地区搜集的183个杨树溃疡病菌(Dothiorella gregaria Sacc.)菌株,在做了培养性状观察和致病力测定的基础上,选取致病力不同的(强、中、弱)18个菌株进行聚丙烯酰胺凝胶电泳,测定酯酶同功酶。结果表明:(1)供试菌株的酯酶同功酶谱大体上可分为2种类型,类型Ⅰ有较完整的显示紫红色和浅褐色2种主酶带,类型Ⅱ缺少或仅具不明显的紫红酶带;(2)菌株紫红色主酶带的多少和颜色深浅与致病力强弱有明显的相关性。

A Study on the Relationship Between the Pathogenicity Differentiation of the Pathogen of Poplar Canker (Dothiorella gregaria Sacc.) and Esterase Isozyme

One handred and eighty three isolates of D.gregaria Sacc.were collected from different species or clones of poplar in Beijing and examined for cultural characteristics and pathogenicity.Eighteen isolates were selected according to pathogenicity (high,middle and weak virulence).The esterase isozymes were then determined using the method of polycrylamide gel electrophorosis.The results are as follows.(1) The esterase isozymes of the tested isolates were divided into two patterns.The first had two main enzyme bounds which were purple.The second had none at all or only unconspicuous purple enzyme bounds; (2) The relationship between the number,colour of enzyme bounds and patho-genicity is evident.