| 百合疫病病原鉴定及其PCR检测 |
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| 引用本文： | 杨秀梅,瞿素萍,王丽花,刘晓侠,彭绿春,王继华.百合疫病病原鉴定及其PCR检测[J].园艺学报,2011,38(6):1180-1186. |
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| 作者姓名： | 杨秀梅 瞿素萍 王丽花 刘晓侠 彭绿春 王继华 |
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| 作者单位： | （1云南省农业科学院花卉研究所，云南省花卉育种重点实验室，昆明 650205；2浙江嘉兴学院生化学院，浙江嘉兴 314001） |
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| 基金项目： | 国家科技支撑计划项目(2007BAD45B01,2007BAD45B04); 云南省自然科学基金项目(2007C121M) |
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| 摘 要： | 对从百合疫病病株上分离得到的病原菌进行了形态特征观察、致病性测定及核糖体DNA-ITS序列分析。结果表明，该菌为疫霉属真菌，与GenBank中烟草疫霉ITS序列的同源性为98% ~ 99%。结合病原菌PCR检测结果，进一步证实其为烟草疫霉菌（Phytophthora nicotianae）。这是烟草疫霉菌侵染云南切花百合的首次报道。
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| 关 键 词： | 百合 疫病 病原鉴定 PCR检测 |
| 收稿时间： | 2011-01-20 |
Identification and PCR Detection of Phytophthora Blight of Lily |
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| YANG Xiu-mei,QU Su-ping,WANG Li-hua,LIU Xiao-xia,PENG Lü-chun,WANG Ji-hua.Identification and PCR Detection of Phytophthora Blight of Lily[J].Acta Horticulturae Sinica,2011,38(6):1180-1186. |
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| Authors: | YANG Xiu-mei QU Su-ping WANG Li-hua LIU Xiao-xia PENG Lü-chun WANG Ji-hua |
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| Institution: | Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325035, China. |
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| Abstract: | The pathogen causing lily Phytophthora blight was isolated from diseased plants with typical symptoms on basal stems.The fungi was identified as Phytophthora nicotianae according to morphological characteristics,pathogenicity and the sequence of ribosomal DNA-ITS.The sequence of ribosomal DNA-ITS of isolate P1 was showed a sequence identity of 98%-99% with Phytophthora nicotianae published in GenBank.PCR detection using P.nicotianae-specific primers indicated that the pathogen was P.nicotianae.This is the f... |
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| Keywords: | lily Phytophthora blight pathogen identification PCR detection |
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