| 纽荷尔脐橙贮藏枯水相关ESTs分离与初步分析 |
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| 引用本文： | 许兰珍,刘小丰,何永睿,姚利晓,彭爱红,雷天刚,陈善春.纽荷尔脐橙贮藏枯水相关ESTs分离与初步分析[J].园艺学报,2011,38(6):1153-1160. |
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| 作者姓名： | 许兰珍 刘小丰 何永睿 姚利晓 彭爱红 雷天刚 陈善春 |
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| 作者单位： | （中国农业科学院/西南大学柑桔研究所，国家柑桔品种改良中心，国家柑桔工程技术研究中心，重庆400712） |
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| 基金项目： | 长江学者和创新团队发展计划项目(PCSIRT); 国家高科技研究发展计划项目(2011AA100205); 柑桔产业技术体系(CARS-27); 农业部公益性行业(农业)科研专项(201003067-02-4); 重庆市科委重点实验室专项经费项目(CSTC) |
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| 摘 要： | 为分析柑橘果实贮藏过程中枯水相关特异基因的表达情况，以纽荷尔脐橙（Citrus Sinensis Osbeck）采摘后贮藏至略见枯水果实的果肉为测验方（Tester），以刚采摘果实的果肉为驱动方（Driver），采用抑制性差减杂交（Suppression Subtractive Hybridization，SSH）技术成功构建了贮藏果实差减cDNA文库，共获得656个阳性克隆。随机挑取克隆进行菌液PCR分析，插入片段长度主要集中在100 ~ 1 000 bp。成功对213个克隆进行测序，获得有效序列143条，经序列分析，共得到53条uniESTs序列。在NCBI基因库中对这53个uniESTs进行BLAST分析，比对结果参照MIPS的分类标准，按功能分为14类，其中参与代谢、能量及蛋白合成的ESTs最多，分别占到了全部ESTs的17%、11%和13%。
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| 关 键 词： | 柑橘 果实 枯水 抑制性差减杂交 EST |
| 收稿时间： | 2010-09-25 |
Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences |
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| XU Lan-zhen,LIU Xiao-feng,HE Yong-rui,YAO Li-xiao,PENG Ai-hong,LEI Tian-gang,CHEN Shan-chun.Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences[J].Acta Horticulturae Sinica,2011,38(6):1153-1160. |
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| Authors: | XU Lan-zhen LIU Xiao-feng HE Yong-rui YAO Li-xiao PENG Ai-hong LEI Tian-gang CHEN Shan-chun |
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| Institution: | （Citrus Research Institute，Chinese Academy of Agricultural Sciences/Southwest University，National Citrus Improvement Center，National Citrus Engineering Research Center，Chongqing 400712，China） |
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| Abstract: | In order to analyze the differentially expressed genes in the post-harvested citrus fruit,a subtracted cDNA library was constructed by suppression subtractive hybridization(SSH)between the fruit pulp of post-storage(experimental group,the Tester)and that of pre-storage(control group,the Driver).The library included about 656 clones which were 100-1 000 bp in size,among which 213 clones were selected randomly and sequenced.Totally 143 useful ESTs were acquired,and 53 uniESTs were finally obtained.Sequence si... |
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| Keywords: | citrus fruit granulation suppression subtractive hybridization EST |
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