H2O2与葡萄VvIPK2基因表达及其低温胁迫响应的关系

 以葡萄砧木品种‘F-242’试管苗为材料，克隆了葡萄（Vitis L.）的多磷酸肌醇激酶（inositol polyphosphate kinase，IPK2）基因VvIPK2，并对其进行生物信息学分析；利用实时定量PCR和植物生理学技术，结合药理学实验探究了H2O2在葡萄响应低温胁迫中的作用及其与VvIPK2的关系。结果表明，克隆的VvIPK2其推导的翻译产物（VvIPK2）与数据库中推测的氨基酸序列一致；与栽培亚麻和拟南芥多磷酸肌醇激酶高度同源。在低温胁迫下，叶片VvIPK2表达与H2O2水平均明显升高；外源H2O2能提高VvIPK2表达水平，而H2O2清除剂抗坏血酸（ascorbic acid，AsA）降低VvIPK2表达水平。外施一定浓度的H2O2可提高超氧化物歧化酶（superoxide dismutase，SOD）活性，上调Cu/Zn SOD基因表达，降低丙二醛（malondialdehyde，MDA）含量和细胞膜相对透性；但IPK2抑制剂LY-294002对内源H2O2却无明显影响；LY-294002和AsA均能降低SOD活性，下调Cu/Zn SOD基因表达，提高MDA含量和细胞膜相对透性。推测H2O2调节VvIPK2的表达，参与葡萄响应低温胁迫过程。

Involvement of H_2O_2 in Regulating the Expression of VvIPK2 in Response to Low Temperature Stress in Leaves of Vitis

An inositol polyphosphate kinase gene named VvIPK2 from Vitis vinifera was cloned and its biomatics was analyzed,the role of H_2O_2 and the interaction between H_2O_2 and VvIPK2 in response to cold stress of'F-242'leaves were investigated by real-time PCR,combined with physiological and pharmacological methods.The results showed that the deduced translation product(VvIPK2)was completely the same with the sequence in database which shares 64% and 62% identity with the inositol polyphosphate kinase(LuIPK2)in ...