| 浑圆链霉菌QH-16鉴定及抑菌活性产物的结构研究 |
| |
| 引用本文： | 李志田,蔡淑琳,王合,杨海青,赵娟,刘德文,刘娅,张殿朋. 浑圆链霉菌QH-16鉴定及抑菌活性产物的结构研究[J]. 中国生物防治学报, 2021, 37(1): 156-164. DOI: 10.16409/j.cnki.2095-039x.2021.01.029 |
| |
| 作者姓名： | 李志田 蔡淑琳 王合 杨海青 赵娟 刘德文 刘娅 张殿朋 |
| |
| 作者单位： | 1. 石河子大学食品学院, 石河子 832003;2. 北京市农林科学院植物保护环境保护研究所, 北京 100097;3. 北京市林业保护站, 北京 100029;4. 北京市平谷区果品办公室, 北京 101200 |
| |
| 基金项目： | 北京市农林科学院科技创新能力建设专项(KJCX20200203,KJCX20180408)。 |
| |
| 摘 要： | 拮抗放线菌能够产生具有较高商业价值的生物活性物质,应用潜力巨大.本研究以桃细菌性穿孔病菌Xanthomonas arboricola pv.pruni为指示菌,采用平板对峙法从已有拮抗菌库中筛选对病原菌具有抑制作用的拮抗放线菌,发现菌株QH?16的拮抗效果最佳,通过16S rDNA序列分析,结合形态特征、培养特性和生理...
|
| 关 键 词： | 桃细菌性穿孔病菌 放线菌 鉴定 活性物质 |
| 收稿时间： | 2020-06-12 |
Identification of Actinomycete Strain QH-16 and Structural Analysis of Its Active Products |
| |
| LI Zhitian,CAI Shulin,WANG He,YANG Haiqing,ZHAO Juan,LIU Dewen,LIU Ya,ZHANG Dianpeng. Identification of Actinomycete Strain QH-16 and Structural Analysis of Its Active Products[J]. Chinese Journal of Biological Control, 2021, 37(1): 156-164. DOI: 10.16409/j.cnki.2095-039x.2021.01.029 |
| |
| Authors: | LI Zhitian CAI Shulin WANG He YANG Haiqing ZHAO Juan LIU Dewen LIU Ya ZHANG Dianpeng |
| |
| Affiliation: | 1. School of Food, Shihezi University, Shihezi 832000, China;2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;3. Forest Pest Management and Quarantine Station of Beijing, Beijing 100029, China;4. Beijing Pinggu District Fruit Office, Beijing 101200, China |
| |
| Abstract: | Antagonistic actinomycetes can produce bioactive compounds with high commercial value and great application potential.In this study,Xanthomonas arboricola pv.pruni was used as the indicator bacteria,and five antagonistic actinomycetes showing inhibition action on pathogen by dual culture assay were screened and strain QH-16 presented the highest antagonistic efficacy.Strain QH-16 was identified by 16S rDNA sequence analysis,morphological observation,cultural and physiological characteristics.The results showed that strain QH-16 was identified as Streptomyces globosus.The filtrate from ferment broth of strain QH-16 could inhibit Fusarium graminearum and Bacillus subtilis,with inhibition bands of 14 mm and 22 mm,respectively.The active ingredients of the fermentation products were extracted by solvent extraction and purified by silica gel column chromatography,thin layer chromatography for preparation(PLC)and preparative analyzed by nuclear magnetic resonanc,the main active product in the fermentation products was identified to be dibutyl phthalate. |
| |
| Keywords: | Xanthomonas arboricola pv.pruni actinomycete identification active product |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国生物防治学报》浏览原始摘要信息 |
|
点击此处可从《中国生物防治学报》下载免费的PDF全文 |
|
